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Search The CSA
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Catalytic Site Atlas

CSA LITERATURE entry for 1w1o

E.C. namecytokinin dehydrogenase
SpeciesZea mays (Maize)
E.C. Number (IntEnz) 1.5.99.12
CSA Homologues of 1w1oThere are 10 Homologs
CSA Entries With UniProtID Q9T0N8
CSA Entries With EC Number 1.5.99.12
PDBe Entry 1w1o
PDBSum Entry 1w1o
MACiE Entry M0115

Literature Report

IntroductionCytokinin dehydrogenase (CKX) from Zea mays catalyses the irreversible oxidation of cytokinins, a type of plant hormone which promotes cell division and differentiation. CKX catalyses the conversion of N6-(delta2-isopentenyl)-adenine into adenine. Increased enzyme activity causes drastic abnormalities in the growth and development of the plant.
Mechansim1. A hydride is accepted by FAD from the substrate C11 atom. 2. A standard FADH oxidation then occurs with quinone. 3. The imine is then hydrolysed in a non-enzymatic step.
Reaction

Catalytic Sites for 1w1o

Annotated By Reference To The Literature - Site 1 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
AspA169169macie:sideChainStabilises the positive charge on the intermediate.
GluA288288macie:sideChainForms a carboxylate-carboxylate pair with Asp 169, facilitating the stabilization of the intermediate by Asp 169.
HisA105105macie:sideChainIs covalently bound to cofactor FAD.

Literature References

Notes:
Malito E
Structures of Michaelis and product complexes of plant cytokinin dehydrogenase: implications for flavoenzyme catalysis.
J Mol Biol 2004 341 1237-1249
PubMed: 15321719
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