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Catalytic Site Atlas

CSA LITERATURE entry for 1m53

E.C. nameisomaltulose synthase
SpeciesKlebsiella sp. lx3 ()
E.C. Number (IntEnz) 5.4.99.11
CSA Homologues of 1m53There are 256 Homologs
CSA Entries With UniProtID
CSA Entries With EC Number 5.4.99.11
PDBe Entry 1m53
PDBSum Entry 1m53
MACiE Entry 1m53

Literature Report

IntroductionIsomaltulose synthase (PalI), also known as sucrose isomerase (EC 5.4.99.11), catalyses the isomerization of sucrose to produce isomaltulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) and trehalulose (alpha-D-glucosylpyranosyl-1,1-D-fructofuranose) as the main products with residual amounts of glucose and fructose Isomaltulose and trehalulose, two functional isomers of sucrose, have been suggested as non-cariogenic alternatives to sucrose and are widely used in health products and the food industry.
MechansimGlu295 protonates the oxygen atom of the glycosidic link, in concert with the nucleophile Asp241 attacking at C1, yielding a molecule of fructose and a glucose-enzyme complex. Sucrose then attacks at C1 with the C6 hydroxyl group, displacing Asp241 to form Isomatulose.
Reaction

Catalytic Sites for 1m53

Literature References

Notes:
Zhang D
Isomaltulose synthase (PalI) of Klebsiella sp. LX3. Crystal structure and implication of mechanism.
J Biol Chem 2003 278 35428-35434
PubMed: 12819210
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