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Catalytic Site Atlas Search Results
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Catalytic Site Atlas

CSA LITERATURE entry for 1jfl

E.C. nameaspartate racemase
SpeciesPyrococcus horikoshii (Bacteria)
E.C. Number (IntEnz) 5.1.1.13
CSA Homologues of 1jfl
CSA Entries With UniProtID
CSA Entries With EC Number 5.1.1.13
PDBe Entry 1jfl
PDBSum Entry 1jfl
MACiE Entry M0335

Literature Report

IntroductionAlthough L-enantiomers of amino acids are predominant in living organisms, D-enantiomers are distributed widely in bacteria, archaea and mammalian tissue. D-aspartate (D-Asp) is one of the most abundant D-enantiomers.
Aspartate racemase (AspR) is a pyridoxal 5'-phosphate (PLP)-independent amino acid racemase that catalyses the interconversion of L-Asp and D-Asp.
MechansimAspR uses a 'two-base' catalytic mechanism, in which a pair of cysteine residues (Cys82 and Cys194) act as the conjugate acid and base. The thiolate of Cys82 deprotonates the alpha-C carbon of the amino acid substrate, resulting in the formation of a carbanionic intermediate, which is reprotonated on the reverse side by Cys194, yielding the product with an inverted configuration to the substrate.
Reaction

Catalytic Sites for 1jfl

Annotated By Reference To The Literature - Site 1 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
CysA8282macie:sideChainCys82 acts as a base, removing a proton from the alpha-C of the substrate to yield the carbanionic intermediate.
CysA194194macie:sideChainCys194 acts as the acid, reprotonating the alpha-C of the carbanionic intermediate on the opposite side to the one that the proton was removed from. This results in the formation of the product with the correct inverted configuration.

Annotated By Reference To The Literature - Site 2 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
CysB8282macie:sideChainCys82 acts as a base, removing a proton from the alpha-C of the substrate to yield the carbanionic intermediate.
CysB194194macie:sideChainCys194 acts as the acid, reprotonating the alpha-C of the carbanionic intermediate on the opposite side to the one that the proton was removed from. This results in the formation of the product with the correct inverted configuration.

Literature References

Notes:
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