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Search The CSA
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Catalytic Site Atlas

CSA LITERATURE entry for 1g4p

E.C. namethiamine-phosphate diphosphorylase
SpeciesBacillus subtilis (Bacteria)
E.C. Number (IntEnz) 2.5.1.3
CSA Homologues of 1g4pThere are 27 Homologs
CSA Entries With UniProtID P39594
CSA Entries With EC Number 2.5.1.3
PDBe Entry 1g4p
PDBSum Entry 1g4p
MACiE Entry 1g4p

Literature Report

IntroductionThiamin phosphate synthase catalyses the formation of thiamin phosphate from 4-amino-5-(hydroxymethyl)-2-methylpyrimidine pyrophosphate and 5-(hydroxyethyl-4-methylthiazole phosphate, in the penultimate step of the biosynthesis of thiamin phosphate.
MechansimThe reaction of thiamin phosphate synthase occurs by a dissociative SN1 mechanism. Ser 130 activates the pyrophosphate as a leaving group and transition state stabilisation is maintained by a magnesium cation. The ionised group is then able to react with 5-(hydroxyethyl-4-methylthiazole phosphate to give thiamin phosphate. The carbocation intermediate is stabilised by the counterion and a hydrogen bond between the pyrimidine amino group and the pyrophosphate.
Reaction

Catalytic Sites for 1g4p

Annotated By Reference To The Literature - Site 1 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
AlaB1130117macie:sideChainActivates the pyrophosphate to act as a leaving group.

Annotated By Reference To The Literature - Site 2 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
AlaA130117macie:sideChainActivates the pyrophosphate to act as a leaving group.

Literature References

Notes:
Peapus DH
Structural characterization of the enzyme-substrate, enzyme-intermediate, and enzyme-product complexes of thiamin phosphate synthase.
Biochemistry 2001 40 10103-10114
PubMed: 11513589
Chiu HJ
Crystal structure of thiamin phosphate synthase from Bacillus subtilis at 1.25 A resolution.
Biochemistry 1999 38 6460-6470
PubMed: 10350464
Reddick JJ
Mechanistic studies on thiamin phosphate synthase: evidence for a dissociative mechanism.
Biochemistry 2001 40 10095-10102
PubMed: 11513588
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