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Catalytic Site Atlas

CSA LITERATURE entry for 1eg7

E.C. nameformate---tetrahydrofolate ligase
SpeciesMoorella thermoacetica (strain ATCC 39073)
E.C. Number (IntEnz) 6.3.4.3
CSA Homologues of 1eg71fp7,1fpm,3do6,
CSA Entries With UniProtID P21164
CSA Entries With EC Number 6.3.4.3
PDBe Entry 1eg7
PDBSum Entry 1eg7
MACiE Entry 1eg7

Literature Report

IntroductionN(10) Formyltetrahydrofolate synthetase (FTHFS) catalyses the ATP dependent activation of formate towards addition at the N10 position of tetrahydrofolate; a reaction of physiological importance to acetogens, catalysing the reduction of carbondioxide and other one carbon containing precursor molecules into acetate.
MechansimFirst, formate acts as a nucleophile towards the gamma phosphate of the Mg-ATP. This formyl phosphate intermediate then acts as an electrophile towards the N10 atom of Tetrahydrofolate (THF), resulting in the formation of 10-formyl-THF. No catalytic residues fitting the CSA definition of catalytic have been identified, although a number of residues involved in binding interactions with the triphosphate and tetrahydrofolate substrates have been determined. The presence of a metal cation enhances catalytic activity, but its binding site is too far from the active site to be implicated in the catalytic mechanism.
Reaction

Catalytic Sites for 1eg7

Literature References

Notes:
Radfar R
The crystal structure of N(10)-formyltetrahydrofolate synthetase from Moorella thermoacetica.
Biochemistry 2000 39 3920-3926
PubMed: 10747779
Mejillano MR
Formation and utilization of formyl phosphate by N10-formyltetrahydrofolate synthetase: evidence for formyl phosphate as an intermediate in the reaction.
Biochemistry 1989 28 5136-5145
PubMed: 2548602
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