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Search The CSA
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Catalytic Site Atlas

CSA LITERATURE entry for 1aw8

E.C. nameaspartate 1-decarboxylase
SpeciesEscherichia coli (Bacteria)
E.C. Number (IntEnz) 4.1.1.11
CSA Homologues of 1aw8There are 13 Homologs
CSA Entries With UniProtID P0A790
CSA Entries With EC Number 4.1.1.11
PDBe Entry 1aw8
PDBSum Entry 1aw8
MACiE Entry 1aw8

Literature Report

IntroductionThis enzyme catalyses the formation of beta-alanine from L-aspartate. This is a step in the biosynthetic pathway of pantothenate, the precursor to phosphopantothenate, the acyl carrier found in coenzyme A and acyl carrier proteins. The enzyme requires a main chain rearrangement of the pro-protein to active enzyme. Autocatalytic post-translational modification which cleaves the Gly24-Ser25 peptide bond and converts Ser25 to a pyruvoyl group yield the active enzyme.
MechansimThe mechanism involves formation of an imine between the amino group of aspartate and an integral pyruvoyl group. The pyruvol group is formed by an autocatalytic post-translational modification which cleaves the Gly24-Ser25 bond and converts Ser25 into the pyruvoyl group.
Aspartate firstly forms an imine-linkage to the pyruvoyl group of the enzyme, followed by the decarboxylation of the aspartate, forming the extended enolate intermediate. The enolate is then reprotonated by Tyr58 to form an imine intermediate of the product, beta-alanine, which is finally released by hydrolysis to regenerate the pyruvoyl group. Lys9 may play a role in keeping the alpha-carboxyl group of the substrate deprotonated by forming an ion pair.
Reaction

Catalytic Sites for 1aw8

Annotated By Reference To The Literature - Site 1 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
LysA99macie:sideChainIt keeps the alpha-carboxyl group of aspartate deprotonated by forming an ion pair.
TyrB5858macie:sideChainIt protonates the enolate intermediate to form an imine intermediate of the product beta-alanine.

Annotated By Reference To The Literature - Site 2 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
TyrE5858macie:sideChainError

Literature References

Notes:
Saldanha SA
Identification of Tyr58 as the proton donor in the aspartate-alpha-decarboxylase reaction.
Chem Commun (Camb) 2001 1760-1761
PubMed: 12240302
Lee BI
Crystal structure of the schiff base intermediate prior to decarboxylation in the catalytic cycle of aspartate alpha-decarboxylase.
J Mol Biol 2004 340 1-7
PubMed: 15184017
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