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Search The CSA
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Catalytic Site Atlas

CSA LITERATURE entry for 1ab8

E.C. nameadenylate cyclase
SpeciesRattus norvegicus (Rat)
E.C. Number (IntEnz) 4.6.1.1
CSA Homologues of 1ab8There are 36 Homologs
CSA Entries With UniProtID P26769
CSA Entries With EC Number 4.6.1.1
PDBe Entry 1ab8
PDBSum Entry 1ab8
MACiE Entry M0058

Literature Report

IntroductionAdenylate cyclase converts adenosine triphosphate (ATP) into cyclic adenosine monophosphate (cAMP), a ubiquitous second messenger that regulates many functions. Many signalling pathways triggered by hormones and other biological processes converge on and regulate the integral membrane enzyme, including those of the heterotrimeric GTP-binding proteins. There are over 30 related proteins, including bacterial and fungal adenylyl cyclases as well as guanylyl cyclases.
MechansimThe alpha-phosphate deprotonates the 3'-hydroxyl of ATP, which in turn attacks the phosphorus atom in a nucleophilic addition, forming a cyclic pentacoordinate phosphorus intermediate. The cyclic pentacoordinate phosphorus intermediate collapses eliminating the diphosphate molecule. One or two magnesium ions have been suggested as playing a part in the catalytic mechanism, for example by stabilising the transition state, but they are not present in the PDB file.
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Catalytic Sites for 1ab8

Annotated By Reference To The Literature - Site 1 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
ArgA10291029macie:sideChainStabilises negatively charged transition state.

Literature References

Notes:
Yan SZ
The conserved asparagine and arginine are essential for catalysis of mammalian adenylyl cyclase.
J Biol Chem 1997 272 12342-12349
PubMed: 9139678
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