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Search The CSA
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Catalytic Site Atlas

CSA LITERATURE entry for 2f9z

E.C. nameprotein-glutamate methylesterase
SpeciesThermotoga maritima msb8 ()
E.C. Number (IntEnz) 3.1.1.61
CSA Homologues of 2f9z
CSA Entries With UniProtID Q9X005
CSA Entries With EC Number 3.1.1.61
PDBe Entry 2f9z
PDBSum Entry 2f9z
MACiE Entry 2f9z

Literature Report

IntroductionCheD is a regulatory protein involved in the signal transduction pathways that contorol bacterial chemotaxis. CheD deamidates receptor glutamine residues contained within a conserved structural motif and also hydrolyses glutamyl-methyl-esters at specific positions on it's target proteins. This pdb (2f9z) contains the structure of the CheC:CheD complex from Thermotoga maritima. CheC is a signal-terminating phosphatase which down regulates CheD activity by mimicking the substrate.
MechansimA mechanism analogous to that seen in cysteine proteases, such as papain, has been proposed for the deamidation. Cys27 acts as the nucleophile. It attacks the carbonyl carbon of the substrate yielding a tetrahedral intermediate. Ammonia is lost yielding a thioester which is hydrolysed by a water molecule.
Reaction

Catalytic Sites for 2f9z

Annotated By Reference To The Literature - Site 1 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
HisC4444macie:sideChainHis44 deprotonates Cys27 so that it may act as nucleophile. His44 may also protonate the amino-leaving group of the substrate.
ThrC2121macie:sideChainStabilises the positions of His44 and Cys27.
CysC2727macie:sideChainCys27 is the nucleophile which attacks the carbonyl carbon of the substrate.

Annotated By Reference To The Literature - Site 2 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
HisD4444macie:sideChainHis44 deprotonates Cys27 so that it may act as nucleophile. His44 may also protonate the amino-leaving group of the substrate.
ThrD2121macie:sideChainStabilises the positions of His44 and Cys27.
CysD2727macie:sideChainCys27 is the nucleophile which attacks the carbonyl carbon of the substrate.

Literature References

Notes:
Chao X
A receptor-modifying deamidase in complex with a signaling phosphatase reveals reciprocal regulation.
Cell 2006 124 561-571
PubMed: 16469702
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