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Search The CSA
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Catalytic Site Atlas

CSA LITERATURE entry for 1tyf

E.C. nameendopeptidase Clp
SpeciesEscherichia coli (Bacteria)
E.C. Number (IntEnz) 3.4.21.92
CSA Homologues of 1tyfThere are 18 Homologs
CSA Entries With UniProtID P0A6G7
CSA Entries With EC Number 3.4.21.92
PDBe Entry 1tyf
PDBSum Entry 1tyf
MACiE Entry 1tyf

Literature Report

Introduction CLP Protease (clpP) is an ATP dependent protease which performs a large proportion of cellular protein degradation in most cells.
clpP is a large tetradecamer (two rings of seven subunits each) which forms a hollow cylinder within which proteins bind and are degraded. ATPase subunit control the activity of the main proteolytic subunits and have also been implicated as having an important role in ensuring correct protein folding.
The fold of the monomer identifies clpP as a member of the crotonase-like superfamily, however it shares no functional similarities with other members of the group. It has been identified as a serine protease, containing a classical catalytic triad however it is structurally unrelated to any of the other serine protease families.
MechansimclpP contains a classic catalytic triad: serine 97 is the nucleophile activated by histidine 122 and aspartate 171. The backbone amides of glycine 68 and methionine 98 form an oxyanion hole.
Reaction

Catalytic Sites for 1tyf

Annotated By Reference To The Literature - Site 1 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetA98112macie:mainChainAmide
SerA97111macie:sideChain
GlyA6882macie:mainChainAmide
HisA122136macie:sideChain
AspA171185macie:sideChain

Annotated By Reference To The Literature - Site 2 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetB98112macie:mainChainAmide
SerB97111macie:sideChain
GlyB6882macie:mainChainAmide
HisB122136macie:sideChain
AspB171185macie:sideChain

Annotated By Reference To The Literature - Site 3 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetC98112macie:mainChainAmide
SerC97111macie:sideChain
GlyC6882macie:mainChainAmide
HisC122136macie:sideChain
AspC171185macie:sideChain

Annotated By Reference To The Literature - Site 4 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetD98112macie:mainChainAmide
SerD97111macie:sideChain
GlyD6882macie:mainChainAmide
HisD122136macie:sideChain
AspD171185macie:sideChain

Annotated By Reference To The Literature - Site 5 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetE98112macie:mainChainAmide
SerE97111macie:sideChain
GlyE6882macie:mainChainAmide
HisE122136macie:sideChain
AspE171185macie:sideChain

Annotated By Reference To The Literature - Site 6 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetF98112macie:mainChainAmide
SerF97111macie:sideChain
GlyF6882macie:mainChainAmide
HisF122136macie:sideChain
AspF171185macie:sideChain

Annotated By Reference To The Literature - Site 7 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetG98112macie:mainChainAmide
SerG97111macie:sideChain
GlyG6882macie:mainChainAmide
HisG122136macie:sideChain
AspG171185macie:sideChain

Annotated By Reference To The Literature - Site 8 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetH98112macie:mainChainAmide
SerH97111macie:sideChain
GlyH6882macie:mainChainAmide
HisH122136macie:sideChain
AspH171185macie:sideChain

Annotated By Reference To The Literature - Site 9 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetI98112macie:mainChainAmide
SerI97111macie:sideChain
GlyI6882macie:mainChainAmide
HisI122136macie:sideChain
AspI171185macie:sideChain

Annotated By Reference To The Literature - Site 10 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetJ98112macie:mainChainAmide
SerJ97111macie:sideChain
GlyJ6882macie:mainChainAmide
HisJ122136macie:sideChain
AspJ171185macie:sideChain

Annotated By Reference To The Literature - Site 11 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetK98112macie:mainChainAmide
SerK97111macie:sideChain
GlyK6882macie:mainChainAmide
HisK122136macie:sideChain
AspK171185macie:sideChain

Annotated By Reference To The Literature - Site 12 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetL98112macie:mainChainAmide
SerL97111macie:sideChain
GlyL6882macie:mainChainAmide
HisL122136macie:sideChain
AspL171185macie:sideChain

Annotated By Reference To The Literature - Site 13 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetM98112macie:mainChainAmide
SerM97111macie:sideChain
GlyM6882macie:mainChainAmide
HisM122136macie:sideChain
AspM171185macie:sideChain

Annotated By Reference To The Literature - Site 14 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
MetN98112macie:mainChainAmide
SerN97111macie:sideChain
GlyN6882macie:mainChainAmide
HisN122136macie:sideChain
AspN171185macie:sideChain

Literature References

Notes:
Wang J
The structure of ClpP at 2.3 A resolution suggests a model for ATP-dependent proteolysis.
Cell 1997 91 447-456
PubMed: 9390554
Wang J
Crystal structure determination of Escherichia coli ClpP starting from an EM-derived mask.
J Struct Biol 1998 124 151-163
PubMed: 10049803
Porankiewicz J
New insights into the ATP-dependent Clp protease: Escherichia coli and beyond.
Mol Microbiol 1999 32 449-458
PubMed: 10320569
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