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Search The CSA
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Catalytic Site Atlas

CSA LITERATURE entry for 1s20

E.C. name3-dehydro-L-gulonate 2-dehydrogenase
SpeciesEscherichia coli (Bacteria)
E.C. Number (IntEnz) 1.1.1.130
CSA Homologues of 1s20There are 13 Homologs
CSA Entries With UniProtID P37672
CSA Entries With EC Number 1.1.1.130
PDBe Entry 1s20
PDBSum Entry 1s20
MACiE Entry 1s20

Literature Report

Introduction2,3-Diketo-L-gulonate reductase (yiaK) is from Escherichia coli. It is the first gene in a nine-gene sequence operon, which is believed to be required for the utilisation of rare sugars for growth. YiaK catalyses the reduction of 2,3-diketo-L-gulonate (DKG) in the presence of NADH to give 3-keto-L-gulonate.
Mechansim1. His 44 protonates the oxygen attached to C2 of the substrate. 2. NADH is the source of a hydride, which nucleophilically attacks C2 of the substrate to give the product, and NAD+.
Reaction

Catalytic Sites for 1s20

Annotated By Reference To The Literature - Site 1 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
HisA4444macie:sideChainHis 44 protonates the oxygen attached to the C2 atom of the substrate.

Annotated By Reference To The Literature - Site 2 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
HisB4444macie:sideChainHis 44 protonates the oxygen attached to the C2 atom of the substrate.

Annotated By Reference To The Literature - Site 3 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
HisC4444macie:sideChainHis 44 protonates the oxygen attached to the C2 atom of the substrate.

Annotated By Reference To The Literature - Site 4 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
HisD4444macie:sideChainHis 44 protonates the oxygen attached to the C2 atom of the substrate.

Annotated By Reference To The Literature - Site 5 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
HisE4444macie:sideChainHis 44 protonates the oxygen attached to the C2 atom of the substrate.

Annotated By Reference To The Literature - Site 6 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
HisF4444macie:sideChainHis 44 protonates the oxygen attached to the C2 atom of the substrate.

Annotated By Reference To The Literature - Site 7 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
HisG4444macie:sideChainHis 44 protonates the oxygen attached to the C2 atom of the substrate.

Annotated By Reference To The Literature - Site 8 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
HisH4444macie:sideChainHis 44 protonates the oxygen attached to the C2 atom of the substrate.

Literature References

Notes:It is possible that His 116 aids His 44 in protonating the substrate.
Forouhar F
A novel NAD-binding protein revealed by the crystal structure of 2,3-diketo-L-gulonate reductase (YiaK).
J Biol Chem 2004 279 13148-13155
PubMed: 14718529
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