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Search The CSA
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Catalytic Site Atlas

CSA LITERATURE entry for 1ofg

E.C. nameglucose-fructose oxidoreductase
SpeciesZymomonas mobilis ()
E.C. Number (IntEnz) 1.1.99.28
CSA Homologues of 1ofgThere are 17 Homologs
CSA Entries With UniProtID Q07982
CSA Entries With EC Number 1.1.99.28
PDBe Entry 1ofg
PDBSum Entry 1ofg
MACiE Entry 1ofg

Literature Report

IntroductionGlucose-fructose oxidoreductase catalyses both the reduction of fructose and the oxidation of glucose simultaneously, reactions which allow certain bacteria to tolerate high sugar concentrations. The gluconolactone produced is subsequently converted to ethanol whilst the sorbital is not metabolised further. The biotechnological interest in an efficient microbial production of ethanol production had lead to a huge interest in enzymes such as these. It is located in the periplasmic space of the bacterial cells where its biological function is to protect the bacterium from osmotic stress caused by high sugar concentrations.
MechansimCatalysis is achieved by a ping pong mechanism where one of its substrates binds and forms a product that dissociates before the second product binds. The residues involved are yet to be identified conclusively but there is some speculation. Tyr217 is the most likely residue to act as a proton donor/acceptor. Lys129 is thought to hydrogen bond to the adjacent Tyr217 and facilitate proton transfer. Other residues that could possibly be implicated in catalysis include Glu128 and Pro130.
Reaction

Catalytic Sites for 1ofg

Annotated By Reference To The Literature - Site 1 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
LysA129181macie:sideChain
TyrA217269macie:sideChain

Annotated By Reference To The Literature - Site 2 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
LysB129181macie:sideChain
TyrB217269macie:sideChain

Annotated By Reference To The Literature - Site 3 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
LysC129181macie:sideChain
TyrC217269macie:sideChain

Annotated By Reference To The Literature - Site 4 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
LysD129181macie:sideChain
TyrD217269macie:sideChain

Annotated By Reference To The Literature - Site 5 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
LysE129181macie:sideChain
TyrE217269macie:sideChain

Annotated By Reference To The Literature - Site 6 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
LysF129181macie:sideChain
TyrF217269macie:sideChain

Literature References

Notes:
Kingston RL.
The structure of glucose-fructose oxidoreductase from Zymomonas mobilis: an osmoprotective periplasmic enzyme containing non-dissociable NADP
Structure 1996 4 1413-1428
PubMed: 8994968
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