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Search The CSA
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Catalytic Site Atlas

CSA LITERATURE entry for 1kyq

E.C. nameprecorrin-2 dehydrogenase
SpeciesSaccharomyces cerevisiae (Baker's yeast)
E.C. Number (IntEnz) 1.3.1.76
CSA Homologues of 1kyq1pjq,1pjs,1pjt,3dfz,
CSA Entries With UniProtID P15807
CSA Entries With EC Number 1.3.1.76
PDBe Entry 1kyq
PDBSum Entry 1kyq
MACiE Entry 1kyq

Literature Report

IntroductionBiosynthesis of the sirohaem cofactor is completed by Met8p, by a beta-NAD+-dependent dehydrogenation of precorrin-2 to produce sirohydrochlorin, followed by ferrochelation to generate sirohaem. Met8p has a similar role in cobalamin biosynthesis.
MechansimNAD-dependent dehydrogenation occurs by Asp 141 functioning as a catalytic general base to abstract a proton from the pyrrole nitrogen of ring C with concomitant hydride transfer from the prochiral bridge carbon to the nicotinamide ring of NAD.
During ferrochelation Asp 141 again acts as a general base to remove additional protons from the pyrrole nitrogens during metal ion insertion.
Reaction

Catalytic Sites for 1kyq

Annotated By Reference To The Literature - Site 1 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
AspA141141macie:sideChainAsp 141 functions as a general base in both dehydrogenase and chelatase reactions by abstraction of protons from pyrrole nitrogens.

Annotated By Reference To The Literature - Site 2 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
AspB141141macie:sideChainAsp 141 functions as a general base in both dehydrogenase and chelatase reactions by abstraction of protons from pyrrole nitrogens.

Annotated By Reference To The Literature - Site 3 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
AspC141141macie:sideChainAsp 141 functions as a general base in both dehydrogenase and chelatase reactions by abstraction of protons from pyrrole nitrogens.

Literature References

Notes:
Schubert HL
The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase.
EMBO J 2002 21 2068-2075
PubMed: 11980703
Leech HK
Production of cobalamin and sirohaem in Bacillus megaterium: an investigation into the role of the branchpoint chelatases sirohydrochlorin ferrochelatase (SirB) and sirohydrochlorin cobalt chelatase (CbiX).
Biochem Soc Trans 2002 30 610-613
PubMed: 12196147
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