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Search The CSA
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Catalytic Site Atlas

CSA LITERATURE entry for 1aui

E.C. namephosphoprotein phosphatase
SpeciesHomo sapiens (Human)
E.C. Number (IntEnz) 3.1.3.16
CSA Homologues of 1auiThere are 43 Homologs
CSA Entries With UniProtID Q08209
CSA Entries With EC Number 3.1.3.16
PDBe Entry 1aui
PDBSum Entry 1aui
MACiE Entry 1aui

Literature Report

IntroductionCalcineurin is a Ca2+- and calmodulin-dependent protein serine/threonine phosphatase. It is important in several Ca2+-dependent eukaryotic signal transduction pathways.
MechansimAn iron-activated water molecule is deprotonated by histidine and carries out a nucleophilic attack on the substrate (a phosphorylated serine or threonine residue). The histidine then donates the proton to the amino acid leaving group.
The 'Asp-His-(OH)-Fe' tetrad is very similar to the 'Asp-His-Ser' triad of serine proteases.
Reaction

Catalytic Sites for 1aui

Annotated By Reference To The Literature - Site 1 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
HisA151151macie:sideChainGeneral acid/base.
ArgA122122macie:sideChainStabilises negatively charged transition state.
ArgA254254macie:sideChainStabilises negatively charged transition state.
AspA121121macie:sideChaintabilises histidine residue when it is positively charged.

Literature References

Notes:
Rusnak F
Calcineurin: form and function.
Physiol Rev 2000 80 1483-1521
PubMed: 11015619
Kissinger CR
Crystal structures of human calcineurin and the human FKBP12-FK506-calcineurin complex.
Nature 1995 378 641-644
PubMed: 8524402
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