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Search The CSA
PDB ID
UNIPROT ID
EC Number

Catalytic Site Atlas

CSA LITERATURE entry for 1ahj

E.C. namenitrile hydratase
SpeciesRhodococcus sp. r312 ()
E.C. Number (IntEnz) 4.2.1.84
CSA Homologues of 1ahjThere are 28 Homologs
CSA Entries With UniProtID P13448
CSA Entries With EC Number 4.2.1.84
PDBe Entry 1ahj
PDBSum Entry 1ahj
MACiE Entry M0057

Literature Report

IntroductionNitrile hydratase catalyses the production of nitriles to the corresponding amides. The enzyme consists of alpha and beta heterodimers. These enzymes have importance as biocatalysts, for example in the production of acrylamide, and in bioremediation.
MechansimUnknown, but involves an iron centre.
Reaction

Catalytic Sites for 1ahj

Annotated By Reference To The Literature - Site 1 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
CysA113113macie:sideChain
SerA114114macie:sideChain
CysA115115macie:sideChain
ArgB5656macie:sideChain

Annotated By Reference To The Literature - Site 2 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
ArgD5656macie:sideChain
CysC113113macie:sideChain
SerC114114macie:sideChain
CysC115115macie:sideChain

Annotated By Reference To The Literature - Site 3 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
ArgF5656macie:sideChain
CysE113113macie:sideChain
SerE114114macie:sideChain
CysE115115macie:sideChain

Annotated By Reference To The Literature - Site 4 (Perform Site Search)
ResidueChainNumberUniProtKB NumberFunctional PartFunctionTargetDescription
ArgH5656macie:sideChain
CysG113113macie:sideChain
SerG114114macie:sideChain
CysG115115macie:sideChain

Literature References

Notes:
Kobayashi M
Nitrile hydrolases.
Curr Opin Chem Biol 2000 4 95-102
PubMed: 10679370
Piersma SR
Arginine 56 mutation in the beta subunit of nitrile hydratase: importance of hydrogen bonding to the non-heme iron center.
J Inorg Biochem 2000 80 283-288
PubMed: 11001100
Huang W
Crystal structure of nitrile hydratase reveals a novel iron centre in a novel fold.
Structure 1997 5 691-699
PubMed: 9195885
Nagashima S
Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms.
Nat Struct Biol 1998 5 347-351
PubMed: 9586994
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