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Catalytic Site Atlas

Enter a PDB code, UniProKB code or EC number in one of the boxes Below to obtain catalytic residue details from the CSA.

Search The CSA
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UNIPROT ID
EC Number

A NEW VERSION OF THE CSA UPDATED 14th November 2013

To reference the CSA please use the latest CSA paper
Furnham N, Holliday GL, de Beer TA, Jacobsen JO, Pearson WR, Thornton JM. The Catalytic Site Atlas 2.0: cataloging catalytic sites and residues identified in enzymes. Nucleic Acids Res. 2014 Jan;42(Database issue):D485-9. PubMed PMID: 24319146.

Introduction

The Catalytic Site Atlas (CSA) is a database documenting enzyme active sites and catalytic residues in enzymes of 3D structure. We defined a classification of catalytic residues which includes only those residues thought to be directly involved in some aspect of the reaction catalysed by an enzyme.
The CSA contains 2 types of entry:

Original hand-annotated entries, derived from the primary literature. References for these entries are given.
Homologous entries, found by sequence comparison methods to one of the original entries. The equivalent residues, which align in sequence to the catalytic residues found in the original entry are documented.

Access to the CSA is via PDB ID, UniProtKB ID or E.C. number. Accessing via PDB ID takes you straight to the CSA entry for that PDB, while accessing via UniProtKB ID or E.C. number gives a list of all PDB codes for structures assigned that particular UniProtKB identifier or E.C. number.

Each CSA entry lists the catalytic residues found in that entry, using PDB residue numbering. Each site is also marked with an evidence tag, which is either "Literature reference" or "Homologue". If the entry is by sequence comparison you can follow the link to the original entry. The active site can be visualised using a JMol viewer and each catalytic site in the structure can be highlighted and zoomed into by selecting from the drop down list on the left hand side of the viewer as well as further rendering options.

Each entry contains a link to a list of homologous entries found by homology, and a link to other PDB structures with identical E.C. numbers or UniProKB identifier to the entry you are viewing.

A number of people have contributed to the CSA over the years as annotators. We would like to thank Jonathan Barker, Carine Berezin, Amy Buchanan-Huges, Lynn Carr, Olivia Chan, Josephine Charalambous, Emma Compton, Atlanta Cook, Jennifer Dawe, Angelica Datta, Christian Drew, Alex Gutteridge, Stephanie Juniat, Roman Laskowski, Oleg Lenive, Mei Leung, Stuart Lucas, Ben McLeod, Malcolm MacArthur, Gary McDowell, Angela Malumbe, Duncan Milburn, Fiona Morgan, James Murray, Nozomi Nagano, Jonathan Ng, Emma Penn, Craig Porter, Judith Reeks, Peter Sarkies, Steven Smith, James Torrance, Annabel Todd, Andrew Wallace, Anna Waters, Sophie Williams, and Eleanor Wright.

References

To reference the CSA please use the latest CSA paper
Furnham N, Holliday GL, de Beer TA, Jacobsen JO, Pearson WR, Thornton JM. The Catalytic Site Atlas 2.0: cataloging catalytic sites and residues identified in enzymes. Nucleic Acids Res. 2014 Jan;42(Database issue):D485-9. PubMed PMID: 24319146.
The Catalytic Site Atlas: a resource of catalytic sites and residues identified in enzymes using structural data. Craig T. Porter, Gail J. Bartlett, and Janet M. Thornton (2004) Nucl. Acids. Res. 32: D129-D133.
Analysis of Catalytic Residues in Enzyme Active Sites. Gail J. Bartlett, Craig T. Porter, Neera Borkakoti, and Janet M. Thornton (2002) J Mol Biol 324:105-121.
Using a Library of Structural Templates to Recognise Catalytic Sites and Explore their Evolution in Homologous Families. James W. Torrance, Gail J. Bartlett, Craig T. Porter, Janet M. Thornton (2005) J Mol Biol. 347:565-81