Enzymes
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Reaction participants Show >> << Hide
- Name help_outline dithioerythritol Identifier CHEBI:17456 (Beilstein: 1719756; CAS: 6892-68-8) help_outline Charge 0 Formula C4H10O2S2 InChIKeyhelp_outline VHJLVAABSRFDPM-ZXZARUISSA-N SMILEShelp_outline O[C@@H](CS)[C@H](O)CS 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline thiosulfate Identifier CHEBI:33542 Charge -1 Formula HO3S2 InChIKeyhelp_outline DHCDFWKWKRSZHF-UHFFFAOYSA-M SMILEShelp_outline [H]SS([O-])(=O)=O 2D coordinates Mol file for the small molecule Search links Involved in 22 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline cis-1,2-dithiane-4,5-diol Identifier CHEBI:28059 (Beilstein: 1280352) help_outline Charge 0 Formula C4H8O2S2 InChIKeyhelp_outline YPGMOWHXEQDBBV-ZXZARUISSA-N SMILEShelp_outline O[C@H]1CSSC[C@H]1O 2D coordinates Mol file for the small molecule Search links Involved in 1 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline H+ Identifier CHEBI:15378 Charge 1 Formula H InChIKeyhelp_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILEShelp_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,176 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline hydrogen sulfide Identifier CHEBI:29919 (CAS: 15035-72-0) help_outline Charge -1 Formula HS InChIKeyhelp_outline RWSOTUBLDIXVET-UHFFFAOYSA-M SMILEShelp_outline [S-][H] 2D coordinates Mol file for the small molecule Search links Involved in 54 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
- Name help_outline sulfite Identifier CHEBI:17359 (CAS: 14265-45-3) help_outline Charge -2 Formula O3S InChIKeyhelp_outline LSNNMFCWUKXFEE-UHFFFAOYSA-L SMILEShelp_outline [O-]S([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 55 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Cross-references
RHEA:15121 | RHEA:15122 | RHEA:15123 | RHEA:15124 | |
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Reaction direction help_outline | undefined | left-to-right | right-to-left | bidirectional |
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Publications
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Isolation and characterization of thiosulfate reductases from the green alga Chlorella fusca.
Schmidt A., Erdle I., Gamon B.
Thiosulfate-reductase activity (TSR) measured as sulfide release from thiosulfate was detected in crude extracts of Chlorella using dithioerythritol (DTE) as electron donor. Purification of this activity by ammonium-sulfate precipitation between 35% and 80% followed by Sephadex G-50 gel filtration ... >> More
Thiosulfate-reductase activity (TSR) measured as sulfide release from thiosulfate was detected in crude extracts of Chlorella using dithioerythritol (DTE) as electron donor. Purification of this activity by ammonium-sulfate precipitation between 35% and 80% followed by Sephadex G-50 gel filtration, diethylaminoethyl-cellulose chromatography, and gel filtration on Biogel A 1.5 M led to four distinct proteins having molecular weights of: TSR I, 28000; TSR II, 26500; TSR IIIa, 55000; TSR IIIb, 24000 daltons. These thiosulfate reductases were most active with DTE; the monothiols glutathione, L-cysteine, and β-mercaptoethanol had little activity towards this system. The following pH optima were obtained: for TSR I and TSR II, 9.0; for TSR IIIa, 8.5; and for TSR IIIb, 9.5. The apparent-Km data for DTE and thiosulfate were determined to: [Formula: see text] TSR I, 0.164 mmol·l(-1) and TSR II, 0.156 mmol·l(-1); KmDTE TSR I, 1.54 mmol·l(-1) and TSR II 1.54 mmol·l(-1). The thiosulfate reductases IIIa and IIIb were further stimulated by addition of thioredoxin. All TSR fractions catalyzed SCN formation from thiosulfate and cyanate and thus had rhodanese activity; this activity, however, could only be detected in the presence of thiols. << Less