Melanoma metastases: association between S100A13 expression and chemotherapy resistance
Here we have performed quantitative profiling of the proteome of lymph node metastasis from patients with melanoma with different response to chemotherapy.
Sample Processing Protocol
Proteins were digested with trypsin, labeled with 8plex iTRAQ and separated by isoelectric focusing before LC-MS/MS on an LTQ Orbitrap Velos.
Data Processing Protocol
MSMS data was searched using Mascot under Proteome Discoverer 1.1 against the human Uniref100 protein sequence database. Variable mods: oxidation of methionine. Fixed mods: carbamidomethylation of cysteine, iTRAQ8plex on N-terminus and on lysine side chains.
Maria Pernemalm, Clinical Proteomics Unit, Dep. of Oncology-Pathology
Janne Lehtiö, Dept. of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden; Clinical proteomics mass spectrometry, Science for Life Laboratory, Sweden. ( lab head )
Azimi A, Pernemalm M, Frostvik Stolt M, Hansson J, Lehtiö J, Egyházi Brage S, Hertzman Johansson C; Proteomics analysis of melanoma metastases: association between S100A13 expression and chemotherapy resistance., Br J Cancer, 2014 Apr 10, PubMed: 24722184