Project PXD000642

Summary

Title

Quantitative proteomics of extracellular vesicles released from human primary macrophages upon beta/glucan stimulation

Description

Fungal infections (mycoses) are common diseases of varying severity that cause problems especially to immunologically compromised people. During stimulatory conditions of infection and colonization, besides intensive intracellular response, human cells actively communicate on the intercellular level by secreting proteins with several mechanisms. The main immunostimulatory components of fungi are beta-glucans, fungal cell-wall components. Here, we have used high-throughput quantitative proteomics combined with bioinformatics to characterize and quantify vesicle-mediated protein release from beta-glucan-stimulated human primary macrophages. We show that β-glucan stimulation induces vesicle-mediated protein secretion. Proteomic study identified 540 distinct proteins from the vesicles including several receptors which have not been identified from vesicles before. Bioinformatics analysis strongly suggests a significant role for these proteins in transfer of biological information. Our current data provides an important resource on the protein composition of extracellular vesicles released from human macrophages upon fungal infection.

Sample Processing Protocol

See details in reference(s) : 24670152

Data Processing Protocol

See details in reference(s) : 24670152

Contact

Tuula Nyman, University of Oslo

Submission Date

13/12/2013

Publication Date

15/04/2014

Tissue

Not available

Instrument

QSTAR

Software

Not available

Experiment Type

Bottom-up proteomics

Publication

    Cypryk W, Ohman T, Eskelinen EL, Matikainen S, Nyman TA. Quantitative proteomics of extracellular vesicles released from human monocyte-derived macrophages upon β-glucan stimulation. J Proteome Res. 2014 Mar 26 PubMed: 24670152