Project : PXD000531

Summary

Title

Human pleural effusion LC-MSMS

Description

We screened pleural effusion proteomes of mesothelioma and lung adenocarcinoma patients to identify novel soluble mesothelioma biomarkers. We performed quantitative mass spectrometry-(MS-) based proteomics using isobaric tags for relative and absolute quantification (iTRAQ) and used narrow range immobilized pH gradient/high resolution isoelectric focusing (IPG/HiRIEF; pH 4 to 4.25) prior to analysis by nano liquid chromatography-coupled MS/MS. Pleural effusions from patients with malignant mesothelioma (n=6), lung adenocarcinoma (n=6), or benign mesotheliosis (n=7) were analyzed, and more than 1,300 proteins were identified.

Sample Processing Protocol

See details in reference PMID : 24361865

Data Processing Protocol

See details in reference PMID : 24361865

Contact

Filip Mundt, Karolinska Institutet

Submission Date

21/10/2013

Tissue

Not available

Instrument

LTQ Orbitrap Velos

Software

Not available

Experiment Type

Bottom-up proteomics

Publication

    Mundt F, Johansson H, Forshed J, Arslan S, Metintas M, Dobra K, Lehtio J, Hjerpe A. Proteome screening of pleural effusions identifies galectin 1 as a diagnostic biomarker and highlights several prognostic biomarkers for malignant mesothelioma. Mol Cell Proteomics. 2013 Dec 20 PubMed(s) : 24361865

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