Project : PXD000472

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Summary

Title

Environmental stress affects the activity of metabolic and growth factor signaling networks and induces autophagy markers in breast cancer cell lines

Description

The breast cancer cell line MCF7 was exposed to RT comditions for 15min, 2h or left in the incubator. Cells were harvested and lyzed. Samples were digested with trypsin and subjected to phosphoenrichment usin TiO2. Phosphopeptides were run in a LTQ-Orbitrap-XL. 4 biological replicates per condition were done. Peaks lists were generated with Mascot Distiller (version 2.3) in MGF format and Database searches were with Mascot Server (version 2.3) against the SwissProt database restricted to human sequences (release October 2012) and trypsin cleavage. Restrictions were 7ppm for parent ions and 0.8 Da for fragment masses. Allowed modifications were phosphorylation of Ser/Thr/Tyr, pyro-Glu (N-term) and methionine oxidation and one miss-cleavage allowed. Quantification was by label-free using peak heights of extracted ion chromatograms (XICs) constructed with narrow mass windows (7ppm) and time windows (1.5 minutes). Pescal, a computer program written in house, was used to automate the generation of XICs and to calculate peak heights.

Sample Processing Protocol

See details in reference PMID : 24425749

Data Processing Protocol

See details in reference PMID : 24425749

Contact

Pedro Casado-Izquierdo, Cell Signalling

Submission Date

18/09/2013

Publication

    Casado P, Bilanges B, Rajeeve V, Vanhaesebroeck B, Cutillas PR. Environmental Stress Affects the Activity of Metabolic and Growth Factor Signaling Networks and Induces Autophagy Markers in MCF7 Breast Cancer Cells. Mol Cell Proteomics. 2014 Mar;13(3):836-48 PubMed(s) : 24425749

Assay

Accession Title Protein Count Peptide Count Unique Peptide Count Spectrum Count Identified Spectrum Count
31307 MCF7 RT Incubatorl 1 641 1482 989 5595 1337
31308 MCF7 RT Incubator 2 598 1473 916 5912 1330
31309 MCF7_RT Incubator 3 640 1533 982 6279 1391
31310 MCF7_RT Incubator 4 616 3118 966 12210 2790
31311 MCF7 RT Room Temperature 2h 1 635 1532 998 6099 1422
31312 MCF7 RT Room Temperature 2h 2 623 1461 929 6034 1323
31313 MCF7_RT Room Temperature 2h 3 710 1710 1075 6174 1527
31314 MCF7_RT Room Temperature 2h 4 659 1677 1013 6576 1502
31315 MCF7 RT Room Temperature 15 min 1 655 1501 1011 6077 1362
31316 MCF7 RT Room Temperature 15 min 2 628 1513 976 6120 1367
31317 MCF7 RT Room Temperature 15 min 3 667 1716 1034 6264 1543
31318 MCF7 RT Room Temperature 15 min 4 606 1553 939 6211 1391

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