Project : PXD000440

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Summary

Title

Qualitatively Different T Cell Phenotypic Responses to IL-2 versus IL-15 are Unified by Identical Dependences on Receptor Signal Strength and Duration

Description

3 biological replicates of F15R-Kit cells stimulated with 500pM IL-2 or IL-15 for 15 minutes, and two biological replicates of unstimulated cells (total 8 conditions) were iTRAQ8plex labeled, combined and analyzed together. Phosphotyrosine peptides were enriched for by immunoprecipitation followed by further enrichment by IMAC. Peptides were anaylzed on an Orbitrap Elite with CID and HCD scans for MS/MS peptide identification.

Sample Processing Protocol

See details in reference PMID : 24298013

Data Processing Protocol

See details in reference PMID : 24298013

Contact

Hannah Johnson, Biological Engineering

Submission Date

02/09/2013

Publication

    Arneja A, Johnson H, Gabrovsek L, Lauffenburger DA, White FM. Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration. J Immunol. 2014 Jan 1;192(1):123-35 PubMed(s) : 24298013

Assay

Accession Title Protein Count Peptide Count Unique Peptide Count Spectrum Count Identified Spectrum Count
31010 iTRAQ8plex labelled F15R-Kit cells stimulated with control (n=2), IL-2 (n=3) or IL15 (n=3); Phosphotyrosine analysis 168 417 195 14477 347

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