Summary

Title

Erythrocyte Proteome & Terminome

Description

Proteins and protein N termini were isolated and enriched from human erythrocytes following the TAILS procedure with heavy dimethyl labeling of free N-termini (Kleifeld et al. Nat Biothech 2010). Following LC-MS/MS analysis on a Thermo Orbitrap XL spectra were matched using Mascot, X!Tandem and MSGF+ databases search engines and trans proteomic pipeline (TPP) modules for statistical evaluation. Maximum peptide level FDR was set to 1% (iProphet probability 0.88) and protein level FDR to 0.7% (proteinProphet probability 0.9).

Sample Processing Protocol

See details in reference PMID : 24555563

Data Processing Protocol

See details in reference PMID : 24555563

Contact

Philipp Lange, Centre for Blood Research

Submission Date

29/08/2013

Publication Date

24/02/2014

Tissue

Not available

Instrument

LTQ Orbitrap

Software

Not available

Experiment Type

Bottom-up proteomics

Publication

    Lange PF, Huesgen PF, Nguyen K, Overall CM. Annotating N termini for the Human Proteome Project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 2014 Feb 21 PubMed(s) : 24555563