Project PXD000119

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Biomedical Dataset
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Summary

Title

Nano HPLC Chip Ion Trap MS/MS of human pancreatic cancer cells

Description

Proteins extracted from Panc1 cells (untreated or treated with ACPA or GW), were analyzed by 2-DE. Modulated proteins were identified by nanoHPLC Chip Ion trap. Bioinformatics pipeline: Mascot search was applied using the MS/MS ion search of Mascot against human entries of the non-redundant NCBI database, with propionamide formation of cysteines as fixed modification and oxidized methionine, acetylated protein N terminus, and phosphorylation of serine, threonine, and tyrosine as variable modifications. Trypsin was specified as the proteolytic enzyme and one missed cleavage was allowed. The mass tolerance of the precursor and fragment ions was set to +- 0.9 Da.

Sample Processing Protocol

See details in reference(s) : 23463621

Data Processing Protocol

See details in reference(s) : 23463621

Contact

Daniela Cecconi, Department of Biotechnology

Submission Date

10/01/2013

Publication Date

10/07/2014

Tissue

Not available

Experiment Type

Bottom-up proteomics

Assay count

59

Publication

    Brandi J, Dando I, Palmieri M, Donadelli M, Cecconi D. Comparative proteomic and phosphoproteomic profiling of pancreatic adenocarcinoma cells treated with CB1 or CB2 agonists. Electrophoresis. 2013 May;34(9-10):1359-68 PubMed: 23463621

Assay

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Showing 1 - 10 of 59 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 28071 nanoHPLC Chip Ion Trap MS/MS 4 10 8 22 9
2 28072 nanoHPLC Chip Ion Trap MS/MS 5 35 26 76 27
3 28073 nanoHPLC Chip Ion Trap MS/MS 2 5 5 14 5
4 28074 nanoHPLC Chip Ion Trap MS/MS 5 17 14 93 16
5 28075 nanoHPLC Chip Ion Trap MS/MS 10 91 23 59 24
6 28076 nanoHPLC Chip Ion Trap MS/MS 2 8 7 180 8
7 28077 nanoHPLC Chip Ion Trap MS/MS 3 12 10 45 10
8 28078 nanoHPLC Chip Ion Trap MS/MS 2 4 4 49 4
9 28079 nanoHPLC Chip Ion Trap MS/MS 6 69 32 85 43
10 28080 nanoHPLC Chip Ion Trap MS/MS 1 12 12 29 12