Summary

Title

In-depth membrane proteomic study of breast cancer tissues

Description

Proteomics on Human breast cancer tissue samples. 9 patients with high risk or 9 patiens low risk MammaPrint breast cancer. Membran fraction. Proteins were reduced, alkylated, digested with Lys-C and Trypsin, and labelled with iTRAQ4plex: 114; high risk; 115; low risk.

Sample Processing Protocol

See details in reference PMID : 23153008

Data Processing Protocol

See details in reference PMID : 23153008

Contact

satoshi muraoka, Proteome Research Center

Submission Date

06/11/2012

Publication Date

08/01/2013

Tissue

Not available

Instrument

LTQ Orbitrap Velos

Software

Not available

Experiment Type

Bottom-up proteomics

Publication

    Muraoka S, Kume H, Adachi J, Shiromizu T, Watanabe S, Masuda T, Ishihama Y, Tomonaga T; In-depth membrane proteomic study of breast cancer tissues for the generation of a chromosome-based protein list., J Proteome Res, 2013 Jan 4, 12, 1, 208-13, PubMed: 23153008