Project : PXD000014

Summary

Title

human 293 cells PEDF LC-MS/MS

Description

Experiment was performed on 293 cells, details are presented in publication: Adenoviral E4 gene stimulates secretion of PEDF that maintains long-term survival of human glomerulus-derived endothelial cells Marina Jerebtsova‡§*, Namita Kumari‖, Yuri Obuhkov‖, and Sergei Nekhai‖†* (to be published in Molecular and Cellular Proteomics), please see more details in paper Workflow HPLC on PicoFrit C18 column, gradient 60 min from 2% to 30% ACN Nanospray 2 kV, flow rate 300 nL/min LTQ Orbitrap MS. Data dependent scan FT MS + 3 FT MS/MS. A blank water run was performed after each sample run Each 24 hours setup performance was tested on Apomyoglobin sample, 12 fmol. Coverage was 39-79 % Search is made by SEQUEST on human database

Sample Processing Protocol

See details in reference PMID : 22915824

Data Processing Protocol

See details in reference PMID : 22915824

Contact

Yuri Obukhov, Department of Medicine

Submission Date

08/08/2012

Tissue

Not available

Instrument

LTQ Orbitrap

Software

Not available

Quantification

Not available

Experiment Type

Bottom-up proteomics

Publication

    Jerebtsova M, Kumari N, Obuhkov Y, Nekhai S. Adenoviral E4 gene stimulates secretion of pigmental epithelium derived factor (PEDF) that maintains long-term survival of human glomerulus-derived endothelial cells. Mol Cell Proteomics. 2012 Nov;11(11):1378-88 PubMed(s) : 22915824

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