Project : PXD000012

View in PRIDE Inspector
Download Project Files

Summary

Title

Resveratrol-induced changes of the human adipocyte secretion profile.

Description

Enlarged white adipose tissue (WAT) is a feature of obesity and leads to changes in its paracrine and endocrine function. Dysfunction of WAT cells is associated with obesity associated disorders like type 2 diabetes and cardiovascular diseases. Resveratrol (RSV) a natural polyphenolic compound mimics beneficial effects of calorie restriction. As such, RSV seems a promising therapeutic target for obesity-associated disorders. The effect of RSV on the human adipokine profile is still elusive. Therefore, a proteomic study together with bioinformatical analysis was performed to investigate the effect of RSV on the secretion profile of mature human Simpson-Golabi-Behmel syndrome (SGBS) adipocytes. RSV incubation resulted in elevated basal glycerol release and reduced intracellular TG content. This increased intracellular lipolysis was accompanied by profound changes in the adipocyte secretion profile. Extracellular matrix proteins were down-regulated while processing proteins were mostly up-regulated after RSV treatment. Interestingly, RSV induced secretion of proteins protective against cellular stress and proteins involved in the regulation of apoptosis. Furthermore, we found a RSV-induced up-regulation of adiponectin and ApoE accompanied by a down-regulation of PAI-1 and PEDF secretion which may improve anti-inflammatory processes and increased insulin sensitivity. These effects are beneficial to alleviate obesity-induced metabolic complications. In addition, two novel RSV-regulated adipocyte-secreted proteins were identified.

Sample Processing Protocol

See details in reference PMID : 22905912

Data Processing Protocol

See details in reference PMID : 22905912

Contact

Jean-Paul Noben, FBI

Submission Date

03/08/2012

Tissue

Not available

Cell Type

fat cell

Instrument

LCQ Duo

Quantification

Not available

Experiment Type

Bottom-up proteomics

Assay count

1

Publication

    Rosenow A, Noben JP, Jocken J, Kallendrusch S, Fischer-Posovszky P, Mariman EC, Renes J; Resveratrol-induced changes of the human adipocyte secretion profile., J Proteome Res, 2012 Sep 7, 11, 9, 4733-43, PubMed(s) : 22905912

Assay

Accession Title Protein Count Peptide Count Unique Peptide Count Spectrum Count Identified Spectrum Count
27081 Resveratrol-induced changes of the human adipocyte secretion profile. 72 424 221 580 424

You are about to run PRIDE Inspector via Java Web Start. Double click on the JNLP file downloaded if it didn't start automatically.

Note: Due to Oracle's recent Java update, you may experience security related problems with Java Web Start. Please add "http://www.ebi.ac.uk" to the URLs listed in the Security tab of your Java Control Panel.