PRIDE > Archive > PRD000250 > 12826

Assay 12826

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Statistics

Protein Count Peptide Count Unique Peptide Count Total Spectrum Count Identified Spectrum Count
241 416 387 416 416

Summary

Title

F25 - Gene expression profile of the Triton X-100 soluble fraction form Bovine brain capillary endothelial cells displaying blood brian barrier phenotype

Short Label

any

Experimental Factor

Not available

Contact

Dr C. FLAHAUT, Physiopathology laboratory of the Blood Brain Barrier, Univserit�� d'Artois, Rue Jean Souvraz, F-62307 LENS, FRANCE

Instrument

Model Source Analyzer Detector
Bruker Ultraflex Ionization Type
ultraFlex TOF/TOF
microchannel plate detector

Additional Details

Name Value
Experiment description Bovine brain capillary endothelial cells (BBCEC) are cocultured in presence of rat astrocytes to reinduce blood brain barrier properties. After checking of BBB propereties reinduction, BBCEC are harvested by collagenase treatment, lysed with Triton X-100 based lysis buffer. Triton X-100 soluble proteins are subjected to organic fractionation step and the 5 generated fractions were submitted to 1D-LC-MS/MS analysisi on MALDI-TOF-TOF-MS.
Original MS data file format Mascot DAT File
Project Triton X-100 reference proteome of brain capillary endothelial cells
XML generation software PRIDE Converter v2.3.3