Project : PRD000250

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Summary

Title

Triton X-100 reference proteome of brain capillary endothelial cells

Description

Bovine brain capillary endothelial cells (BBCEC) are cocultured in presence of rat astrocytes to reinduce blood brain barrier properties. After checking of BBB propereties reinduction, BBCEC are harvested by collagenase treatment, lysed with Triton X-100 based lysis buffer. Triton X-100 soluble proteins are subjected to organic fractionation step and the 5 generated fractions were submitted to 1D-LC-MS/MS analysisi on MALDI-TOF-TOF-MS.

Sample Processing Protocol

Not available

Data Processing Protocol

Not available

Contact

Christophe FLAHAUT, Physiopathology laboratory of the Blood Brain Barrier

Submission Date

27/05/2010

Publication

Publication pending

Assay

Accession Title Protein Count Peptide Count Unique Peptide Count Spectrum Count Identified Spectrum Count
12825 F0 - Gene expression profile of the Triton X-100 soluble fraction form Bovine brain capillary endothelial cells displaying blood brian barrier phenotype 144 248 243 248 248
12826 F25 - Gene expression profile of the Triton X-100 soluble fraction form Bovine brain capillary endothelial cells displaying blood brian barrier phenotype 241 416 387 416 416
12827 F50 - Gene expression profile of the Triton X-100 soluble fraction form Bovine brain capillary endothelial cells displaying blood brian barrier phenotype 195 341 341 341 341
12828 F75 - Gene expression profile of the Triton X-100 soluble fraction form Bovine brain capillary endothelial cells displaying blood brian barrier phenotype 193 363 360 363 363
12829 F100 - Gene expression profile of the Triton X-100 soluble fraction form Bovine brain capillary endothelial cells displaying blood brian barrier phenotype 9 15 15 15 15
12830 F 0 to 100 - Gene expression profile of the Triton X-100 soluble fraction form Bovine brain capillary endothelial cells displaying blood brian barrier phenotype 389 1385 750 1385 1385

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