Project PRD000227

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Summary

Title

Eucalyptus globulus-root proteome

Description

Eucalyptus globulus (Labill.) is used for pulp and paper production worldwide. In this report we studied changes in protein expression in one osmotically stressed elite clone widely used in industrial plantations in Spain. High molecular weight polyethylene glycol (PEG) was used as an osmoticum in the growing medium. Roots of rooted cuttings were sampled after 3 and 36 hours of treatment. Water potential and abscissic acid content were measured in shoot and root apices to characterize the physiological states of the plants. Total soluble proteins from roots were extracted and separated using two-dimensional gel electrophoresis (2-DE). Gels were stained with Coomassie brillant blue for quantitative analysis of protein accumulation. From a total of 406 reproducible spots, 34 were found to be differentially expressed depending on treatment (osmotic versus control condition) and/or stress duration (3h versus 36h), and were further characterized by tandem mass spectrometry. Several proteins were reliably identified including adenosine kinase, actin, stress-related proteins as well as proteins associated to cellular processes, among which some residents of the endoplasmic reticulum. This study constitutes the first investigation of the root proteome in this important forest tree genus.

Sample Processing Protocol

See details in reference PMID : 20974537

Data Processing Protocol

See details in reference PMID : 20974537

Contact

Frank Bedon, National Institute for Agricultural Research

Submission Date

20/05/2010

Publication Date

21/10/2010

Modification

Not available

Quantification

Not available

Experiment Type

Bottom-up proteomics

Assay count

34

Publication

    Bedon F, Majada J, Feito I, Chaumeil P, Dupuy JW, Lomenech AM, Barre A, Gion JM, Plomion C; Interaction between environmental factors affects the accumulation of root proteins in hydroponically grown Eucalyptus globulus (Labill.)., Plant Physiol Biochem, 2011 Jan, 49, 1, 69-76, PubMed: 20974537

Assay

Page 1 2 3 4
Page size 10 20
Showing 1 - 10 of 34 results
# Accession Title Proteins Peptides Unique Peptides Spectra Identified Spectra View in Reactome
1 12141 Root proteome in Eucalyptus 73 122 116 122 122
2 12142 Root proteome in Eucalyptus 77 131 128 131 131
3 12143 Root proteome in Eucalyptus 69 119 114 119 119
4 12144 Root proteome in Eucalyptus 67 117 113 117 117
5 12145 Root proteome in Eucalyptus 78 143 139 143 143
6 12146 Root proteome in Eucalyptus 89 132 131 132 132
7 12147 Root proteome in Eucalyptus 89 126 125 126 126
8 12148 Root proteome in Eucalyptus 76 123 120 123 123
9 12149 Root proteome in Eucalyptus 75 127 124 127 127
10 12150 Root proteome in Eucalyptus 77 120 115 120 120