PDBsum entry 6vqh

Proton transport

PDB id: 6vqh

Contents

Protein chains

104 a.a.

110 a.a.

441 a.a.

203 a.a.

41 a.a.

350 a.a.

78 a.a.

84 a.a.

(+ 3 more) 150 a.a.

52 a.a.

PDB id:

6vqh

Name:

Proton transport

Title:

Mammalian v-atpase from rat brain membrane-embedded vo region rotational state 3 (from focused refinement)

Structure:

Atpase h+-transporting v1 subunit d. Chain: h. Synonym: atpase,h+ transporting,v1 subunit d,isoform cra_c,lysosomal v1 subunit d. V-type proton atpase subunit f. Chain: l. Synonym: v-atpase subunit f,v-atpase 14 kda subunit,vacuolar proton pump subunit f. V-type proton atpase 116 kda subunit a isoform 1.

Source:

Rattus norvegicus. Rat. Organism_taxid: 10116. Organism_taxid: 10116

Authors:

Y.M.Abbas,J.L.Rubinstein

Key ref:

Y.M.Abbas et al. (2020). Structure of V-ATPase from the mammalian brain. Science, 367, 1240-1246. PubMed id: 32165585 DOI: 10.1126/science.aaz2924

Date:

05-Feb-20 Release date: 18-Mar-20

Protein chain

Q6P503  (Q6P503_RAT) -  V-type proton ATPase subunit D from Rattus norvegicus

Seq: 247 a.a.

Struc: 104 a.a.

Protein chain

P50408  (VATF_RAT) -  V-type proton ATPase subunit F from Rattus norvegicus

Seq: 119 a.a.

Struc: 110 a.a.

Protein chain

P25286  (VPP1_RAT) -  V-type proton ATPase 116 kDa subunit a 1 from Rattus norvegicus

Seq: 838 a.a.

Struc: 441 a.a.*

Protein chain

B0K022  (B0K022_RAT) -  ATPase, H+ transporting, V0 subunit B (Predicted), isoform CRA_a from Rattus norvegicus

Seq: 205 a.a.

Struc: 203 a.a.

Protein chain

O54715  (VAS1_RAT) -  V-type proton ATPase subunit S1 from Rattus norvegicus

Seq: 463 a.a.

Struc: 41 a.a.

Protein chain

Q5M7T6  (Q5M7T6_RAT) -  V-type proton ATPase subunit from Rattus norvegicus

Seq: 351 a.a.

Struc: 350 a.a.

Protein chain

Q5EB76  (VA0E2_RAT) -  V-type proton ATPase subunit e 2 from Rattus norvegicus

Seq: 81 a.a.

Struc: 78 a.a.

Protein chain

D3ZIM6  (D3ZIM6_RAT) -  Similar to RIKEN cDNA 1110020A23 from Rattus norvegicus

Seq: 71 a.a.

Struc: 84 a.a.

Protein chains

P63081  (VATL_RAT) -  V-type proton ATPase 16 kDa proteolipid subunit c from Rattus norvegicus

Seq: 155 a.a.

Struc: 150 a.a.

Protein chain

Q6AXS4  (RENR_RAT) -  Renin receptor from Rattus norvegicus

Seq: 350 a.a.

Struc: 52 a.a.

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

DOI no: 10.1126/science.aaz2924

Science 367:1240-1246 (2020)

PubMed id: 32165585

Structure of V-ATPase from the mammalian brain.

Y.M.Abbas, D.Wu, S.A.Bueler, C.V.Robinson, J.L.Rubinstein.

ABSTRACT

In neurons, the loading of neurotransmitters into synaptic vesicles uses energy from proton-pumping vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases). These membrane protein complexes possess numerous subunit isoforms, which complicates their analysis. We isolated homogeneous rat brain V-ATPase through its interaction with SidK, a Legionella pneumophila effector protein. Cryo-electron microscopy allowed the construction of an atomic model, defining the enzyme's ATP:proton ratio as 3:10 and revealing a homolog of yeast subunit f in the membrane region, which we tentatively identify as RNAseK. The c ring encloses the transmembrane anchors for cleaved ATP6AP1/Ac45 and ATP6AP2/PRR, the latter of which is the (pro)renin receptor that, in other contexts, is involved in both Wnt signaling and the renin-angiotensin system that regulates blood pressure. This structure shows how ATP6AP1/Ac45 and ATP6AP2/PRR enable assembly of the enzyme's catalytic and membrane regions.