PDBsum entry 6v8f

Lyase

PDB id: 6v8f

Contents

Protein chains

462 a.a.

Ligands

SO4 ×2

GOL ×2

Metals

_CL ×2

Waters ×603

PDB id:

6v8f

Name:

Lyase

Title:

Crystal structure of recombinat mutant q185r of human fumarase

Structure:

Fumarate hydratase, mitochondrial. Chain: a, b. Synonym: hsfh. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: fh. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.30Å R-factor: 0.152 R-free: 0.181

Authors:

M.A.A.Ajalla,M.C.Nonato

Key ref:

M.A.A.Ajalla and m.c.nonato Crystal structure of recombinat mutant q185r of human fumarase. To be published, .

Date:

11-Dec-19 Release date: 16-Dec-20

Protein chains

P07954  (FUMH_HUMAN) -  Fumarate hydratase, mitochondrial from Homo sapiens

Seq: 510 a.a.

Struc: 462 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.2.1.2 - fumarate hydratase.
• Pathway: Citric acid cycle
• Reaction: (S)-malate = fumarate + H2O

(S)-malate (Bound ligand (Het Group name = GOL) matches with 50.00% similarity) = fumarate + H2O

Molecule diagrams generated from .mol files obtained from the KEGG ftp site