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PDBsum entry 6v2s
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Gene regulation
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PDB id
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6v2s
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PDB id:
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Gene regulation
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Title:
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Crystal structure of chromodomain of mpp8 in complex with inhibitor unc3866
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Structure:
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M-phase phosphoprotein 8. Chain: a, b. Fragment: chromodomain. Synonym: two hybrid-associated protein 3 with ranbpm,twa3. Engineered: yes. Unc3866. Chain: c, d. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: mphosph8, mpp8. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: -v2r-prare2. Synthetic: yes. Synthetic construct.
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Resolution:
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1.60Å
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R-factor:
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0.210
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R-free:
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0.243
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Authors:
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Y.Liu,W.Tempel,J.R.Walker,C.Bountra,C.H.Arrowsmith,A.M.Edwards,J.Min, Structural Genomics Consortium (Sgc)
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Key ref:
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C.Dong
et al.
(2020).
Structural Basis for the Binding Selectivity of Human CDY Chromodomains.
Cell Chem Biol,
27,
827.
PubMed id:
DOI:
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Date:
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25-Nov-19
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Release date:
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25-Dec-19
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PROCHECK
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Headers
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References
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Q99549
(MPP8_HUMAN) -
M-phase phosphoprotein 8 from Homo sapiens
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Seq:
Struc:
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860 a.a.
60 a.a.
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DOI no:
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Cell Chem Biol
27:827
(2020)
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PubMed id:
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Structural Basis for the Binding Selectivity of Human CDY Chromodomains.
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C.Dong,
Y.Liu,
T.J.Lyu,
S.Beldar,
K.N.Lamb,
W.Tempel,
Y.Li,
Z.Li,
L.I.James,
S.Qin,
Y.Wang,
J.Min.
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ABSTRACT
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The CDY (chromodomain on the Y) proteins play an essential role in normal
spermatogenesis and brain development. Dysregulation of their expression has
been linked to male infertility and various neurological diseases. Like the
chromodomains of HP1 and Polycomb, the CDY chromodomains also recognize the
lysine-methylated ARKS motif embedded in histone and non-histone proteins.
Interestingly, the CDY chromodomains exhibit different binding preferences for
the lysine-methylated ARKS motif in different sequence contexts. Here, we
present the structural basis for selective binding of CDY1 to H3K9me3 and
preferential binding of CDYL2 to H3tK27me3 over H3K27me3. In addition, we use a
CDYL1/2-selective compound, UNC4850, to gain further insight into the molecular
mechanisms underlying CDYL2 binding specificity. Our work also provides critical
implications that CDYL1b's role in the regulation of neural development is
dependent on its recognition of the lysine-methylated ARKS motif.
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Links
