PDBsum entry 6qnh

Isomerase

PDB id: 6qnh

Contents

Protein chain

387 a.a.

Metals

_CO

_MG

Waters ×255

PDB id:

6qnh

Name:

Isomerase

Title:

Liquid application method for time-resolved analyses (lama) by serial synchrotron crystallography, xylose isomerase 0ms timepoint

Structure:

Xylose isomerase. Chain: a. Engineered: yes

Source:

Streptomyces rubiginosus. Organism_taxid: 1929. Gene: xyla. Expressed in: streptomyces rubiginosus. Expression_system_taxid: 1929

Resolution:

1.85Å R-factor: 0.166 R-free: 0.196

Authors:

P.Mehrabi,E.C.Schulz,R.J.D.Miller

Key ref:

P.Mehrabi et al. (2019). Liquid application method for time-resolved analyses by serial synchrotron crystallography. Nat Methods, 16, 979-982. PubMed id: 31527838 DOI: 10.1038/s41592-019-0553-1

Date:

11-Feb-19 Release date: 02-Oct-19

Protein chain

P24300  (XYLA_STRRU) -  Xylose isomerase from Streptomyces rubiginosus

Seq: 388 a.a.

Struc: 387 a.a.

Enzyme reactions

• Enzyme class: E.C.5.3.1.5 - xylose isomerase.
• Reaction: alpha-D-xylose = alpha-D-xylulofuranose
• Cofactor: Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1038/s41592-019-0553-1

Nat Methods 16:979-982 (2019)

PubMed id: 31527838

Liquid application method for time-resolved analyses by serial synchrotron crystallography.

P.Mehrabi, E.C.Schulz, M.Agthe, S.Horrell, G.Bourenkov, D.von Stetten, J.P.Leimkohl, H.Schikora, T.R.Schneider, A.R.Pearson, F.Tellkamp, R.J.D.Miller.

ABSTRACT

We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.