PDBsum entry 6i2h

Transferase

PDB id: 6i2h

Contents

Protein chains

353 a.a.

18 a.a.

Ligands

AO8

RIP

Metals

_CL

Waters ×196

PDB id:

6i2h

Name:

Transferase

Title:

Crystal structure of the protein-kinase a catalytic subunit from cricetulus griseus in complex with compounds rkp182 and rkp190

Structure:

Camp-dependent protein kinase catalytic subunit alpha. Chain: a. Synonym: pka c-alpha. Engineered: yes. Upf0418 protein fam164a. Chain: d. Engineered: yes. Other_details: pki 5-22 with g17rbs mutation. A ribose has been covalently attached to a serine of the peptide

Source:

Cricetulus griseus. Chinese hamster. Organism_taxid: 10029. Organ: ovary. Tissue: ovary. Gene: prkaca. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768. Synthetic: yes.

Resolution:

1.68Å R-factor: 0.182 R-free: 0.202

Authors:

J.M.Mueller,A.Heine,G.Klebe

Key ref:

J.M.Mueller et al. Crystal structure of the protein-Kinase a catalytic s from cricetulus griseus in complex with compounds rkp rkp190. To be published, .

Date:

01-Nov-18 Release date: 20-Nov-19

Supersedes:

6eme

Protein chain

P25321  (KAPCA_CRIGR) -  cAMP-dependent protein kinase catalytic subunit alpha from Cricetulus griseus

Seq: 351 a.a.

Struc: 353 a.a.*

Protein chain

G3HK48  (G3HK48_CRIGR) -  UPF0418 protein FAM164A from Cricetulus griseus

Seq: 241 a.a.

Struc: 18 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.7.11.11 - cAMP-dependent protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site