PDBsum entry 6h0c

Oxidoreductase

PDB id: 6h0c

Contents

Protein chain

406 a.a.

Ligands

FLC

Metals

_CL

Waters ×344

PDB id:

6h0c

Name:

Oxidoreductase

Title:

Flv1 flavodiiron core from synechocystis sp. Pcc6803

Structure:

Putative diflavin flavoprotein a 3. Chain: a. Engineered: yes

Source:

Synechocystis sp. (Strain pcc 6803 / kazusa). Organism_taxid: 1111708. Strain: pcc 6803 / kazusa. Gene: dfa3, sll1521. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.59Å R-factor: 0.148 R-free: 0.148

Authors:

P.T.Borges,C.V.Romao,L.Saraiva,V.L.Goncalves,M.A.Carrondo,M.Teixeira, C.Frazao

Key ref:

P.T.Borges et al. (2019). Analysis of a new flavodiiron core structural arrangement in Flv1-ΔFlR protein from Synechocystis sp. PCC6803. J Struct Biol, 205, 91. PubMed id: 30447285 DOI: 10.1016/j.jsb.2018.11.004

Date:

08-Jul-18 Release date: 30-Jan-19

Protein chain

P74373  (DFA3_SYNY3) -  Putative diflavin flavoprotein A 3 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)

Seq: 597 a.a.

Struc: 406 a.a.

Enzyme reactions

• Enzyme class: E.C.1.-.-.-

DOI no: 10.1016/j.jsb.2018.11.004

J Struct Biol 205:91 (2019)

PubMed id: 30447285

Analysis of a new flavodiiron core structural arrangement in Flv1-ΔFlR protein from Synechocystis sp. PCC6803.

P.T.Borges, C.V.Romão, L.M.Saraiva, V.L.Gonçalves, M.A.Carrondo, M.Teixeira, C.Frazão.

ABSTRACT

Flavodiiron proteins (FDPs) play key roles in biological response mechanisms against oxygen and/or nitric oxide; in particular they are present in oxygenic phototrophs (including cyanobacteria and gymnosperms). Two conserved domains define the core of this family of proteins: a N-terminal metallo-β-lactamase-like domain followed by a C-terminal flavodoxin-like one, containing the catalytic diiron centre and a FMN cofactor, respectively. Members of the FDP family may present extra modules in the C-terminus, and were classified into several classes according to their distribution and composition. The cyanobacterium Synechocystis sp. PCC6803 contains four Class C FDPs (Flv1-4) that include at the C-terminus an additional NAD(P)H:flavin oxidoreductase (FlR) domain. Two of them (Flv3 and Flv4) have the canonical diiron ligands (Class C, Type 1), while the other two (Flv1 and Flv2) present different residues in that region (Class C, Type 2). Most phototrophs, either Bacterial or Eukaryal, contain at least two FDP genes, each encoding for one of those two types. Crystals of the Flv1 two core domains (Flv1-ΔFlR), without the C-terminal NAD(P)H:flavin oxidoreductase extension, were obtained and the structure was determined. Its pseudo diiron site contains non-canonical basic and neutral residues, and showed anion moieties, instead. The presented structure revealed for the first time the structure of the two-domain core of a Class C-Type 2 FDP.