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PDBsum entry 6gdh
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Recombination
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PDB id
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6gdh
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PDB id:
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| Name: |
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Recombination
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Title:
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Holliday junctions formed from telomeric DNA
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Structure:
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DNA (5'-d( Cp Tp Ap Ap Cp Cp Cp Tp Ap A)-3'). Chain: a, c, e, g. Engineered: yes. DNA (5'-d( Tp Tp Ap Gp Gp Gp Tp Tp Ap G)-3'). Chain: b, d, f, h. Engineered: yes
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Source:
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Synthetic: yes. Homo sapiens. Human. Organism_taxid: 9606. Organism_taxid: 9606
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Resolution:
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2.85Å
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R-factor:
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0.230
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R-free:
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0.273
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Authors:
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G.N.Parkinson,S.Haider,P.Li,S.Khiali,D.Munnur,A.Ramanathan
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Key ref:
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S.Haider
et al.
(2018).
Holliday Junctions Formed from Human Telomeric DNA.
J Am Chem Soc,
140,
15366-15374.
PubMed id:
DOI:
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Date:
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23-Apr-18
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Release date:
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07-Nov-18
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Headers
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References
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C-T-A-A-C-C-C-T-A-A
10 bases
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T-T-A-G-G-G-T-T-A-G
10 bases
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C-T-A-A-C-C-C-T-A-A
10 bases
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T-T-A-G-G-G-T-T-A-G
10 bases
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C-T-A-A-C-C-C-T-A-A
10 bases
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T-T-A-G-G-G-T-T-A-G
10 bases
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C-T-A-A-C-C-C-T-A-A
10 bases
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T-T-A-G-G-G-T-T-A-G
10 bases
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DOI no:
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J Am Chem Soc
140:15366-15374
(2018)
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PubMed id:
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Holliday Junctions Formed from Human Telomeric DNA.
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S.Haider,
P.Li,
S.Khiali,
D.Munnur,
A.Ramanathan,
G.N.Parkinson.
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ABSTRACT
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Cells have evolved inherent mechanisms, like homologous recombination (HR), to
repair damaged DNA. However, repairs at telomeres can lead to genomic
instability, often associated with cancer. While most rapidly dividing cells
employ telomerase, the others maintain telomere length through HR-dependent
alternative lengthening of telomeres (ALT) pathways. Here we describe the
crystal structures of Holliday junction intermediates of the HR-dependent ALT
mechanism. Using an extended human telomeric repeat, we also report the crystal
structure of two Holliday junctions in close proximity, which associate together
through strand exchange to form a hemicatenated double Holliday junction. Our
combined structural results demonstrate that ACC nucleotides in the C-rich
lagging strand (5'-CTAACCCTAA-3') at the telomere repeat sequence constitute a
conserved structural feature that constrains crossover geometry and is a
preferred site for Holliday junction formation in telomeres.
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Links
