PDBsum entry 6fid

Hydrolase

PDB id: 6fid

Contents

Protein chain

223 a.a.

Ligands

BEN

SO4 ×3

Metals

_CA

Waters ×170

PDB id:

6fid

Name:

Hydrolase

Title:

Bovine trypsin solved by s-sad on id30b

Structure:

Cationic trypsin. Chain: a. Synonym: beta-trypsin. Engineered: yes

Source:

Bos taurus. Bovine. Organism_taxid: 9913. Expressed in: bos taurus. Expression_system_taxid: 9913

Resolution:

2.20Å R-factor: 0.176 R-free: 0.220

Authors:

A.A.Mccarthy,C.Mueller-Dieckmann

Key ref:

A.A.McCarthy et al. (2018). ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF. J Synchrotron Radiat, 25, 1249-1260. PubMed id: 29979188

Date:

18-Jan-18 Release date: 07-Feb-18

Protein chain

P00760  (TRY1_BOVIN) -  Serine protease 1 from Bos taurus

Seq: 246 a.a.

Struc: 223 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.21.4 - trypsin.
• Reaction: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

J Synchrotron Radiat 25:1249-1260 (2018)

PubMed id: 29979188

ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF.

A.A.McCarthy, R.Barrett, A.Beteva, H.Caserotto, F.Dobias, F.Felisaz, T.Giraud, M.Guijarro, R.Janocha, A.Khadrouche, M.Lentini, G.A.Leonard, M.Lopez Marrero, S.Malbet-Monaco, S.McSweeney, D.Nurizzo, G.Papp, C.Rossi, J.Sinoir, C.Sorez, J.Surr, O.Svensson, U.Zander, F.Cipriani, P.Theveneau, C.Mueller-Dieckmann.

ABSTRACT

ID30B is an undulator-based high-intensity, energy-tuneable (6.0-20 keV) and variable-focus (20-200 µm in diameter) macromolecular crystallography (MX) beamline at the ESRF. It was the last of the ESRF Structural Biology Group's beamlines to be constructed and commissioned as part of the ESRF's Phase I Upgrade Program and has been in user operation since June 2015. Both a modified microdiffractometer (MD2S) incorporating an in situ plate screening capability and a new flexible sample changer (the FlexHCD) were specifically developed for ID30B. Here, the authors provide the current beamline characteristics and detail how different types of MX experiments can be performed on ID30B (http://www.esrf.eu/id30b).