PDBsum entry 6f4z

DNA

PDB id: 6f4z

Contents

DNA/RNA

PDB id:

6f4z

Name:

DNA

Title:

2'f-arag modified quadruplex with flipped g-tract and central tetrad

Structure:

DNA (5'-d( Gp Gp Gp Ap Tp Gp Gp Gp Ap Cp Ap Cp Ap Gp (Gfl) p Gp Gp Ap Cp Gp Gp G)-3'). Chain: a. Engineered: yes

Source:

Synthetic: yes. Synthetic construct. Organism_taxid: 32630

NMR struc:

10 models

Authors:

J.Dickerhoff,K.Weisz

Key ref:

J.Dickerhoff and K.Weisz (2018). Fluorine-Mediated Editing of a G-Quadruplex Folding Pathway. Chembiochem, 19, 927-930. PubMed id: 29460996 DOI: 10.1002/cbic.201800099

Date:

30-Nov-17 Release date: 25-Apr-18

DNA/RNA chain

G-G-G-A-T-G-G-G-A-C-A-C-A-G-GFL-G-G-A-C-G-G-G 22 bases

DOI no: 10.1002/cbic.201800099

Chembiochem 19:927-930 (2018)

PubMed id: 29460996

Fluorine-Mediated Editing of a G-Quadruplex Folding Pathway.

J.Dickerhoff, K.Weisz.

ABSTRACT

A (3+1)-hybrid-type G-quadruplex was substituted within its central tetrad by a single 2'-fluoro-modified guanosine. Driven by the anti-favoring nucleoside analogue, a novel quadruplex fold with inversion of a single G-tract and conversion of a propeller loop into a lateral loop emerges. In addition, scalar couplings across hydrogen bonds demonstrate the formation of intra- and inter-residual F⋅⋅⋅H8-C8 pseudo-hydrogen bonds within the modified quadruplexes. Alternative folding can be rationalized by the impact of fluorine on intermediate species on the basis of a kinetic partitioning mechanism. Apparently, chemical or other environmental perturbations are able to redirect folding of a quadruplex, possibly modulating its regulatory role in physiological processes.