PDBsum entry 5u7c

Lyase,metal binding protein

PDB id: 5u7c

Contents

Protein chains

208 a.a.

Ligands

ACT ×2

Metals

ZN7 ×2

_BR

Waters ×298

PDB id:

5u7c

Name:

Lyase,metal binding protein

Title:

Crystal structure of the lead-bound form of merb formed from diethyllead.

Structure:

Alkylmercury lyase. Chain: a, b. Synonym: organomercurial lyase. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: merb. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.75Å R-factor: 0.172 R-free: 0.203

Authors:

H.M.Wahba,M.Stevenson,A.Mansour,J.Sygusch,D.E.Wilcox,J.G.Omichinski

Key ref:

H.M.Wahba et al. (2017). Structural and Biochemical Characterization of Organotin and Organolead Compounds Binding to the Organomercurial Lyase MerB Provide New Insights into Its Mechanism of Carbon-Metal Bond Cleavage. J Am Chem Soc, 139, 910-921. PubMed id: 27989130 DOI: 10.1021/jacs.6b11327

Date:

12-Dec-16 Release date: 11-Jan-17

Protein chains

P77072  (MERB_ECOLX) -  Alkylmercury lyase from Escherichia coli

Seq: 212 a.a.

Struc: 209 a.a.

Enzyme reactions

• Enzyme class: E.C.4.99.1.2 - alkylmercury lyase.
• Reaction: an alkylmercury + H⁺ = an alkane + Hg²⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1021/jacs.6b11327

J Am Chem Soc 139:910-921 (2017)

PubMed id: 27989130

Structural and Biochemical Characterization of Organotin and Organolead Compounds Binding to the Organomercurial Lyase MerB Provide New Insights into Its Mechanism of Carbon-Metal Bond Cleavage.

H.M.Wahba, M.J.Stevenson, A.Mansour, J.Sygusch, D.E.Wilcox, J.G.Omichinski.

ABSTRACT

No abstract given.