PDBsum entry 5tpx

Transferase

PDB id: 5tpx

Contents

Protein chain

106 a.a.

Ligands

SO4 ×3

7H7

Metals

_CL

Waters ×66

PDB id:

5tpx

Name:

Transferase

Title:

Bromodomain from plasmodium faciparum gcn5, complexed with compound

Structure:

Histone acetyltransferase gcn5. Chain: a. Fragment: bromodomain (unp residues 1356-1460). Engineered: yes

Source:

Plasmodium falciparum (isolate 3d7). Organism_taxid: 36329. Strain: isolate 3d7. Gene: pf3d7_0823300. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.10Å R-factor: 0.188 R-free: 0.236

Authors:

Y.H.Lin,C.F.D.Hou,M.Moustakim,D.J.Dixon,P.Loppnau,W.Tempel,C.Bountra, A.M.Edwards,C.H.Arrowsmith,R.Hui,P.E.Brennan,J.R.Walker,Structural Genomics Consortium (Sgc)

Key ref:

M.Moustakim et al. (2017). Discovery of a PCAF Bromodomain Chemical Probe. Angew Chem Int Ed Engl, 56, 827-831. PubMed id: 27966810 DOI: 10.1002/anie.201610816

Date:

21-Oct-16 Release date: 04-Jan-17

Protein chain

Q8IB67  (Q8IB67_PLAF7) -  Histone acetyltransferase GCN5 from Plasmodium falciparum (isolate 3D7)

Seq: 1465 a.a.

Struc: 106 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.3.1.48 - histone acetyltransferase.
• Reaction: L-lysyl-[protein] + acetyl-CoA = N₆-acetyl-L-lysyl-[protein] + CoA + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1002/anie.201610816

Angew Chem Int Ed Engl 56:827-831 (2017)

PubMed id: 27966810

Discovery of a PCAF Bromodomain Chemical Probe.

M.Moustakim, P.G.Clark, L.Trulli, A.L.Fuentes de Arriba, M.T.Ehebauer, A.Chaikuad, E.J.Murphy, J.Mendez-Johnson, D.Daniels, C.D.Hou, Y.H.Lin, J.R.Walker, R.Hui, H.Yang, L.Dorrell, C.M.Rogers, O.P.Monteiro, O.Fedorov, K.V.Huber, S.Knapp, J.Heer, D.J.Dixon, P.E.Brennan.

ABSTRACT

No abstract given.