PDBsum entry 5t5n

Membrane protein

PDB id: 5t5n

Contents

Protein chains

366 a.a.

212 a.a.

211 a.a.

Ligands

GOL ×5

Metals

__K ×5

_CL ×15

_CA ×5

Waters ×10

PDB id:

5t5n

Name:

Membrane protein

Title:

Calcium-activated chloride channel bestrophin-1 (best1), triple mutant: i76a, f80a, f84a; in complex with an fab antibody fragment, chloride, and calcium

Structure:

Bestrophin-1 (best1). Chain: a, b, c, d, e. Engineered: yes. Mutation: yes. Fab antibody fragment, light chain (10d10). Chain: f, h, j, l, n. Fab antibody fragment, heavy chain (10d10). Chain: g, i, k, m, o

Source:

Gallus gallus. Chicken. Organism_taxid: 9031. Expressed in: pichia. Expression_system_taxid: 4919. Mus musculus. Mouse. Organism_taxid: 10090. Cell_line: hybridoma.

Resolution:

3.10Å R-factor: 0.218 R-free: 0.243

Authors:

S.B.Long,G.Vaisey

Key ref:

G.Vaisey et al. (2016). Distinct regions that control ion selectivity and calcium-dependent activation in the bestrophin ion channel. Proc Natl Acad Sci U S A, 113, E7399. PubMed id: 27821745 DOI: 10.1073/pnas.1614688113

Date:

31-Aug-16 Release date: 30-Nov-16

Protein chains

E1C3A0  (E1C3A0_CHICK) -

Protein chains

No UniProt id for this chain

Struc: 212 a.a.

Protein chains

No UniProt id for this chain

Struc: 211 a.a.

DOI no: 10.1073/pnas.1614688113

Proc Natl Acad Sci U S A 113:E7399 (2016)

PubMed id: 27821745

Distinct regions that control ion selectivity and calcium-dependent activation in the bestrophin ion channel.

G.Vaisey, A.N.Miller, S.B.Long.

ABSTRACT

No abstract given.