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PDBsum entry 5o2y
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Protein transport
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PDB id
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5o2y
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PDB id:
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| Name: |
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Protein transport
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Title:
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Nmr structure of the calcium bound form of pulg, major pseudopilin from klebsiella oxytoca t2ss
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Structure:
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General secretion pathway protein g. Chain: a. Synonym: general secretion pathway protein gspg. Engineered: yes
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Source:
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Klebsiella oxytoca. Organism_taxid: 571. Gene: pulg, ab185_31145, samea2273639_02747. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008
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NMR struc:
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15 models
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Authors:
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A.Lopez-Castilla,B.Bardiaux,B.Vitorge,J.-L.Thomassin,W.Zheng,X.Yu, E.H.Egelman,M.Nilges,O.Francetic,N.Izadi-Pruneyre
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Key ref:
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A.López-Castilla
et al.
(2017).
Structure of the calcium-dependent type 2 secretion pseudopilus.
Nat Microbiol,
2,
1686-1695.
PubMed id:
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Date:
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23-May-17
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Release date:
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18-Oct-17
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PROCHECK
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Headers
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References
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Nat Microbiol
2:1686-1695
(2017)
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PubMed id:
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Structure of the calcium-dependent type 2 secretion pseudopilus.
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A.López-Castilla,
J.L.Thomassin,
B.Bardiaux,
W.Zheng,
M.Nivaskumar,
X.Yu,
M.Nilges,
E.H.Egelman,
N.Izadi-Pruneyre,
O.Francetic.
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ABSTRACT
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Many Gram-negative bacteria use type 2 secretion systems (T2SSs) to secrete
proteins involved in virulence and adaptation. Transport of folded proteins via
T2SS nanomachines requires the assembly of inner membrane-anchored fibres called
pseudopili. Although efficient pseudopilus assembly is essential for protein
secretion, structure-based functional analyses are required to unravel the
mechanistic link between these processes. Here, we report an atomic model for a
T2SS pseudopilus from Klebsiella oxytoca, obtained by fitting the NMR structure
of its calcium-bound subunit PulG into the ~5-Å-resolution cryo-electron
microscopy reconstruction of assembled fibres. This structure reveals the
comprehensive network of inter-subunit contacts and unexpected features,
including a disordered central region of the PulG helical stem, and highly
flexible C-terminal residues on the fibre surface. NMR, mutagenesis and
functional analyses highlight the key role of calcium in PulG folding and
stability. Fibre disassembly in the absence of calcium provides a basis for
pseudopilus length control, essential for protein secretion, and supports the
Archimedes screw model for the type 2 secretion mechanism.
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Links
