PDBsum entry 5o2b

Structural genomics

PDB id: 5o2b

Contents

Protein chains

269 a.a.

Ligands

KS1 ×2

Metals

_NA

Waters ×252

PDB id:

5o2b

Name:

Structural genomics

Title:

Crystal structure of wnk3 kinase domain in a diphosphorylated state and in a complex with the inhibitor pp-121

Structure:

Serine/threonine-protein kinase wnk3. Chain: a, b. Fragment: unp residues 132-414. Synonym: protein kinase lysine-deficient 3,protein kinase with no lysine 3. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: wnk3, kiaa1566, prkwnk3. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108

Resolution:

2.04Å R-factor: 0.201 R-free: 0.240

Authors:

D.M.Pinkas,J.C.Bufton,J.A.Newman,O.Borkowska,R.Chalk,N.A.Burgess- Brown,F.Von Delft,C.H.Arrowsmith,A.M.Edwards,C.Bountra,A.Bullock, Structural Genomics Consortium (Sgc)

Key ref:

D.M.Pinkas et al. Crystal structure of wnk3 kinase domain in a diphosphorylated state and in a complex with the inhi pp-121. To be published, .

Date:

19-May-17 Release date: 28-Jun-17

Protein chains

Q9BYP7  (WNK3_HUMAN) -  Serine/threonine-protein kinase WNK3 from Homo sapiens

Seq: 1800 a.a.

Struc: 269 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site