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PDBsum entry 5mla
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protein ligands metals Protein-protein interface(s) links
Hydrolase PDB id
5mla

 

 

 

 

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JSmol PyMol  
Contents
Protein chains
164 a.a.
142 a.a.
Ligands
GSP
SO4 ×2
Metals
_MG
Waters ×85
PDB id:
5mla
Name: Hydrolase
Title: Crystal structure of human ras in complex with darpin k55
Structure: Gtpase kras. Chain: a. Synonym: k-ras 2,ki-ras,c-k-ras,c-ki-ras. Engineered: yes. Darpin k55. Chain: b. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: kras, kras2, rask2. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic construct. Organism_taxid: 32630. Expression_system_taxid: 562
Resolution:
2.19Å     R-factor:   0.184     R-free:   0.229
Authors: J.E.Debreczeni,S.Guillard,P.Kolasinska-Zwierz,J.Breed,J.Zhang,N.Bery, R.Marwood,J.Tart,R.Overman,P.Stocki,B.Mistry,C.Phillips,T.Rabbitts, R.Jackson,R.Minter
Key ref: S.Guillard et al. Inhibition of ras nucleotide exchange by a darpin: structural characterisation and effects on downstream signalling by active ras. To be published, .
Date:
06-Dec-16     Release date:   20-Dec-17    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P01116  (RASK_HUMAN) -  GTPase KRas from Homo sapiens
Seq:
Struc: 		189 a.a.
164 a.a.*
Protein chain
No UniProt id for this chain
Struc: 142 a.a.
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chain A: E.C.3.6.5.2  - small monomeric GTPase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: GTP + H2O = GDP + phosphate + H+
GTP
Bound ligand (Het Group name = GSP)
matches with 93.94% similarity 		+ H2O
 = GDP
 + phosphate
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

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