PDBsum entry 5ieb

Protein

PDB id: 5ieb

Contents

Protein chain

118 a.a.

PDB id:

5ieb

Name:

Protein

Title:

Solution structure of sdrg from sphingomonas melonis fr1

Structure:

Sensory transduction regulatory protein. Chain: a. Engineered: yes

Source:

Sphingomonas melonis fr1. Organism_taxid: 1090317. Gene: sr41_04275. Expressed in: escherichia coli. Expression_system_taxid: 562

NMR struc:

20 models

Authors:

S.Campagne,J.A.Vorholt,F.H.-T.Allain

Key ref:

S.Campagne et al. (2016). Role of the PFXFATG[G/Y] Motif in the Activation of SdrG, a Response Regulator Involved in the Alphaproteobacterial General Stress Response. Structure, 24, 1237-1247. PubMed id: 27396826 DOI: 10.1016/j.str.2016.05.015

Date:

25-Feb-16 Release date: 20-Jul-16

Protein chain

A0A0D1MA58  (A0A0D1MA58_9SPHN) -  Sensory transduction regulatory protein from Sphingomonas melonis

Seq: 116 a.a.

Struc: 118 a.a.

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1016/j.str.2016.05.015

Structure 24:1237-1247 (2016)

PubMed id: 27396826

Role of the PFXFATG[G/Y] Motif in the Activation of SdrG, a Response Regulator Involved in the Alphaproteobacterial General Stress Response.

S.Campagne, S.Dintner, L.Gottschlich, M.Thibault, M.Bortfeld-Miller, A.Kaczmarczyk, A.Francez-Charlot, F.H.Allain, J.A.Vorholt.

ABSTRACT

Two-component systems are major signal transduction pathways, which consist of histidine kinases and response regulators that communicate through phosphorylation. Here, we highlight a distinct class of single-domain response regulators containing the PFXFATG[G/Y] motif that are activated by a mechanism distinct from the Y-T coupling described for prototypical receiver domains. We first solved the structures of inactive and active SdrG, a representative of the FAT GUY family, and then biochemically and genetically characterized variants in which residues in this motif were mutated. Our results support a model of activation mainly driven by a conserved lysine and reveal that the rotation of the threonine induces the reorganization of several aromatic residues in and around the PFXFATG[G/Y] motif to generate intermediates resembling those occurring during classical Y-T coupling. Overall, this helps define a new subfamily of response regulators that emerge as important players in physiological adaptation.