PDBsum entry 5fos

Hydrolase

PDB id: 5fos

Contents

Protein chains

225 a.a.

16 a.a.

Ligands

PO4

GOL ×4

Waters ×290

PDB id:

5fos

Name:

Hydrolase

Title:

Humanised monomeric rada in complex with oligomerisation peptide

Structure:

DNA repair and recombination protein rada. Chain: a. Fragment: atpase, unp residues 108-349. Synonym: rada recombinase. Engineered: yes. Mutation: yes. DNA repair and recombination protein rada. Chain: c. Fragment: oligomerisation peptide, unp residues 93-108.

Source:

Pyrococcus furiosus. Organism_taxid: 2261. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: pubs520. Synthetic: yes. Organism_taxid: 2261

Resolution:

1.35Å R-factor: 0.143 R-free: 0.182

Authors:

T.Sharpe,T.Moschetti,G.Fischer,M.Marsh,T.L.Blundell,C.Abell,M.Hyvonen

Key ref:

T.Moschetti et al. (2016). Engineering Archeal Surrogate Systems for the Development of Protein-Protein Interaction Inhibitors against Human RAD51. J Mol Biol, 428, 4589-4607. PubMed id: 27725183 DOI: 10.1016/j.jmb.2016.10.009

Date:

26-Nov-15 Release date: 19-Oct-16

Protein chain

O74036  (RADA_PYRFU) -  DNA repair and recombination protein RadA from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Seq: 349 a.a.

Struc: 225 a.a.*

Protein chain

O74036  (RADA_PYRFU) -  DNA repair and recombination protein RadA from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Seq: 349 a.a.

Struc: 16 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, C: E.C.3.6.4.- - ?????

DOI no: 10.1016/j.jmb.2016.10.009

J Mol Biol 428:4589-4607 (2016)

PubMed id: 27725183

Engineering Archeal Surrogate Systems for the Development of Protein-Protein Interaction Inhibitors against Human RAD51.

T.Moschetti, T.Sharpe, G.Fischer, M.E.Marsh, H.K.Ng, M.Morgan, D.E.Scott, T.L.Blundell, A.R Venkitaraman, J.Skidmore, C.Abell, M.Hyvönen.

ABSTRACT

No abstract given.