PDBsum entry 4zgk

Ligase

PDB id: 4zgk

Contents

Protein chains

94 a.a.

Ligands

4NX ×2

Waters ×114

PDB id:

4zgk

Name:

Ligase

Title:

Structure of mdm2 with low molecular weight inhibitor.

Structure:

E3 ubiquitin-protein ligase mdm2. Chain: a, b. Fragment: unp residues 18-114. Synonym: double minute 2 protein,hdm2,oncoprotein mdm2,p53-binding protein mdm2. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mdm2. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693

Resolution:

2.00Å R-factor: 0.191 R-free: 0.247

Authors:

A.Twarda-Clapa,K.M.Zak,E.M.Wrona,P.Grudnik,G.Dubin,T.A.Holak

Key ref:

E.Surmiak et al. (2016). A Unique Mdm2-Binding Mode of the 3-Pyrrolin-2-one- and 2-Furanone-Based Antagonists of the p53-Mdm2 Interaction. Acs Chem Biol, 11, 3310-3318. PubMed id: 27709883 DOI: 10.1021/acschembio.6b00596

Date:

23-Apr-15 Release date: 19-Oct-16

Protein chains

Q00987  (MDM2_HUMAN) -  E3 ubiquitin-protein ligase Mdm2 from Homo sapiens

Seq: 491 a.a.

Struc: 94 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine

DOI no: 10.1021/acschembio.6b00596

Acs Chem Biol 11:3310-3318 (2016)

PubMed id: 27709883

A Unique Mdm2-Binding Mode of the 3-Pyrrolin-2-one- and 2-Furanone-Based Antagonists of the p53-Mdm2 Interaction.

E.Surmiak, A.Twarda-Clapa, K.M.Zak, B.Musielak, M.D.Tomala, K.Kubica, P.Grudnik, M.Madej, M.Jablonski, J.Potempa, J.Kalinowska-Tluscik, A.Dömling, G.Dubin, T.A.Holak.

ABSTRACT

No abstract given.