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PDBsum entry 4zah
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PDB id:
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Transferase
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Title:
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Crystal structure of sugar aminotransferase wece with external aldimine vii from escherichia coli k-12
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Structure:
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Dtdp-4-amino-4,6-dideoxygalactose transaminase. Chain: a, b, c, d, e, f, g, h. Synonym: lipopolysaccharide biosynthesis protein rffa. Engineered: yes
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Source:
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Escherichia coli (strain k12). Organism_taxid: 83333. Strain: k12. Gene: rffa, wece, yifi, b3791, jw3765. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768.
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Resolution:
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2.24Å
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R-factor:
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0.204
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R-free:
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0.246
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Authors:
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F.Wang,S.Singh,H.Cao,W.Xu,M.D.Miller,J.S.Thorson,G.N.Phillips Jr., Enzyme Discovery For Natural Product Biosynthesis (Natpro)
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Key ref:
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F.Wang
et al.
(2015).
Structural Basis for the Stereochemical Control of Amine Installation in Nucleotide Sugar Aminotransferases.
Acs Chem Biol,
10,
2048-2056.
PubMed id:
DOI:
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Date:
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13-Apr-15
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Release date:
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29-Apr-15
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Supersedes:
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PROCHECK
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Headers
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References
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P27833
(WECE_ECOLI) -
dTDP-4-amino-4,6-dideoxygalactose transaminase from Escherichia coli (strain K12)
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Seq:
Struc:
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376 a.a.
370 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Enzyme class:
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E.C.2.6.1.59
- dTDP-4-amino-4,6-dideoxygalactose transaminase.
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Reaction:
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dTDP-4-amino-4,6-dideoxy-alpha-D-galactose + 2-oxoglutarate = dTDP-4- dehydro-6-deoxy-alpha-D-glucose + L-glutamate
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dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
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+
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2-oxoglutarate
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=
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dTDP-4- dehydro-6-deoxy-alpha-D-glucose
Bound ligand (Het Group name = )
matches with 66.67% similarity
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+
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L-glutamate
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Cofactor:
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Pyridoxal 5'-phosphate
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Pyridoxal 5'-phosphate
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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DOI no:
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Acs Chem Biol
10:2048-2056
(2015)
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PubMed id:
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Structural Basis for the Stereochemical Control of Amine Installation in Nucleotide Sugar Aminotransferases.
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F.Wang,
S.Singh,
W.Xu,
K.E.Helmich,
M.D.Miller,
H.Cao,
C.A.Bingman,
J.S.Thorson,
G.N.Phillips.
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ABSTRACT
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Links
