PDBsum entry 4z4h

Gene regulation/RNA

PDB id: 4z4h

Contents

Protein chain

802 a.a.

DNA/RNA

Ligands

IPH ×4

Metals

_MG ×3

Waters ×135

PDB id:

4z4h

Name:

Gene regulation/RNA

Title:

Human argonaute2 a481t mutant bound to t1-a target RNA

Structure:

Protein argonaute-2. Chain: a. Synonym: hago2,argonaute risc catalytic component 2,eukaryotic translation initiation factor 2c 2,eif2c 2,paz piwi domain protein, ppd,protein slicer. Engineered: yes. Mutation: yes. RNA (5'- r(p Up Up Cp Ap Cp Ap Up Up Gp Cp Cp Cp Ap Ap Gp Up Cp Up Up U)-3').

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ago2, eif2c2. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf-9. Synthetic: yes. Synthetic construct.

Resolution:

2.50Å R-factor: 0.174 R-free: 0.211

Authors:

N.T.Schirle,I.J.Macrae

Key ref:

N.T.Schirle et al. (2015). Water-Mediated recognition of t1-Adenosine anchors argonaute2 to microrna targets.. Elife, 4, . PubMed id: 26359634

Date:

02-Apr-15 Release date: 23-Sep-15

Protein chain

Q9UKV8  (AGO2_HUMAN) -  Protein argonaute-2 from Homo sapiens

Seq: 859 a.a.

Struc: 802 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

U-U-C-A-C-A-U-U-G-C-C-C-A-A-G-U-C-U-U-U 20 bases

C-A-A-U-G-U-G-A 8 bases

Enzyme reactions

• Enzyme class: E.C.3.1.26.n2 - ?????