PDBsum entry 4z2c

Isomerase

PDB id: 4z2c

Contents

Protein chains

485 a.a.

198 a.a.

DNA/RNA

Ligands

MFX ×2

Metals

_MG ×2

Waters ×9

PDB id:

4z2c

Name:

Isomerase

Title:

Quinolone(moxifloxacin)-DNA cleavage complex of gyrase from s. Pneumoniae

Structure:

DNA gyrase subunit a. Chain: a, b. Engineered: yes. DNA gyrase subunit b. Chain: c, d. Engineered: yes. Symmetrized e-site DNA. Chain: e, g. Engineered: yes.

Source:

Streptococcus pneumoniae. Organism_taxid: 1313. Gene: gyra, bm52_0349. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: gyrb, bm52_1967, dj38_04430. Synthetic: yes. Escherichia coli. Organism_taxid: 562.

Resolution:

3.19Å R-factor: 0.226 R-free: 0.268

Authors:

I.Laponogov,D.A.Veselkov,X.-S.Pan,J.Selvarajah,I.M.-T.Crevel, L.M.Fisher,M.R.Sanderson

Key ref:

I.Laponogov et al. Structural studies of the drug-Stabilized cleavage co of topoisomerase IV and gyrase from streptococcus pne. To be published, .

Date:

29-Mar-15 Release date: 14-Sep-16

Protein chains

Q8DPM2  (GYRA_STRR6) -  DNA gyrase subunit A from Streptococcus pneumoniae (strain ATCC BAA-255 / R6)

Seq: 822 a.a.

Struc: 485 a.a.

Protein chains

P0A4M0  (GYRB_STRR6) -  DNA gyrase subunit B from Streptococcus pneumoniae (strain ATCC BAA-255 / R6)

Seq: 648 a.a.

Struc: 198 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chains

A-C-G-T-T-G-T-A-T 9 bases

G-A-T-C-A-T-A-C-A-A-C-G-T 13 bases

G-A-T-C-A-T-A-C-A-A-C 11 bases

G-T-T-G-T-A-T 7 bases

Enzyme reactions

• Enzyme class: Chains A, C, B, D: E.C.5.6.2.2 - Dna topoisomerase (ATP-hydrolyzing).